| 广东凉茶提取物对兔红细胞膜氧化损伤保护作用研究 |
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| 引用本文： | 张可欣,黄鹭,黄晓晖,赵敏,杨杏芬,朱焕容,张梦梦,谭剑斌. 广东凉茶提取物对兔红细胞膜氧化损伤保护作用研究[J]. 华南预防医学, 2019, 45(1): 21-25. DOI: 10.13217/j.scjpm.2019.0021 |
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| 作者姓名： | 张可欣 黄鹭 黄晓晖 赵敏 杨杏芬 朱焕容 张梦梦 谭剑斌 |
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| 作者单位： | 广东省疾病预防控制中心,广东广州511430;中山大学公共卫生学院;广东省疾病预防控制中心,广东广州,511430;广东药科大学;南方医科大学;广州王老吉药业股份有限公司 |
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| 基金项目： | 广州市科技计划项目（201803010105）; 广东省医学科研基金（A2017017） |
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| 摘 要： | 目的从膜成分、结构和功能角度观察广东凉茶提取物对兔红细胞膜氧化损伤的保护作用。方法实验设对照组、H2O2组(1 000μmol/L)、0.1 mg组(0.1 mg/mL凉茶+H2O2)、0.2 mg组(0.2 mg/mL凉茶+H2O2)、0.4 mg组(0.4 mg/mL凉茶+H2O2),参照Dodge法制备兔红细胞膜,观察各组对丙二醛(MDA)含量、蛋白羰基含量、膜封闭能力、细胞膜ATP酶活力、谷胱甘肽过氧化物酶(GPX)活力、过氧化氢酶(CAT)活力和细胞膜蛋白成分的影响。结果与对照组相比,H2O2组蛋白羰基含量、GPX酶活力升高,细胞膜封闭率、CAT酶活力、Ca2+Mg2+?ATP、Mg2+?ATP、Na+K+?ATP酶活力降低(均P<0.01)。与H2O2组比,广东凉茶提取物0.1 mg组、0.2 mg组、0.4 mg组的MDA和DNPH含量均降低,细胞膜封闭率、Ca2+Mg2+?ATP、Mg2+?ATP、Na+K+?ATP酶活力、GPX酶活力、CAT酶活力升高,差异均有统计学意义(均P<0.05)。结论广东凉茶提取物可抑制H2O2引起的红细胞膜成分、结构和功能的损伤作用。
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| 关 键 词： | 广东凉茶 红细胞膜 氧化损伤 抗氧化剂 膜封闭度 |
| 收稿时间： | 2018-08-01 |
Protective effect of Guangdong herbal tea extract on oxidative damage of rabbit erythrocyte membrane |
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| ZHANG Ke-xin,HUANG Lu,HUANG Xiao-hui,ZHAO Min,YANG Xing-fen,ZHU Huan-rong,ZHANG Meng-meng,TAN Jian-bin. Protective effect of Guangdong herbal tea extract on oxidative damage of rabbit erythrocyte membrane[J]. South China JOurnal of Preventive Medicine, 2019, 45(1): 21-25. DOI: 10.13217/j.scjpm.2019.0021 |
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| Authors: | ZHANG Ke-xin HUANG Lu HUANG Xiao-hui ZHAO Min YANG Xing-fen ZHU Huan-rong ZHANG Meng-meng TAN Jian-bin |
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| Affiliation: | (Guangdong Provincial Center for Disease Control and Prevention,Guangzhou 511430,China;School of Public Health,Sun Yat-sen University;Guangdong Pharmaceutical University;Southern Medical University;Guangzhou WanglaoJi Pharmaceutical Company Limited) |
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| Abstract: | Objective To observe the protective effect of Guangdong herbal tea extract on oxidative damage of rabbit erythrocyte membrane from the perspective of membrane composition, structure and function. Methods The experiment consisted of a control group, H2O2 group （1 000 μmol/L）, 0.1 mg group （0.1 mg/ml herbal tea +1 000 μmol/L H202）, 0.2 mg group （0.2 mg/mL herbal tea +1 000 μmol/L H202）, and 0.4 mg group （0.4 mg/mL herbal tea +1 000 μmol/L H202）. Rabbit red blood cell membrane was prepared by Dodge method. The effects of malondialdehyde （MDA）, the carbonyl content of protein, membrane blocking ability, ATPase activity, glutathione peroxidase （GPX）, catalase （CAT） activity, and membrane protein composition on each group were observed. Results Compared with the control group, the carbonyl content of protein and GPX activity were increased, while cell membrane blocking ability, activity of CAT, Ca2+Mg2+-ATPase, Mg2+-ATPase, and Na+K+-ATPase in H2O2 group were decreased （P < 0.01 for all）. Compared with the H2O2 group, contents of MDA and DNPH decreased, while the cell membrane blocking rate, and activities of Ca2+Mg2+-ATPase, Mg2+-ATPase, Na+K+-ATPase, GPX, and CAT increased in the three herbal tea groups （P < 0.05 for all）. Conclusion Guangdong herbal tea extract could inhibit the damage of erythrocyte membrane composition, structure and function caused by hydrogen peroxide. |
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| Keywords: | Guangdong herbal tea Erythrocyte membrane Oxidative damage Antioxidant Blocking degree |
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