| 牙本质涎磷蛋白、Ⅰ型胶原蛋白在vps4b基因 敲除鼠磨牙牙胚发育中的时空表达 |
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| 引用本文： | 陈栋,王莹莹,李晓聪,鲁方丽,李强. 牙本质涎磷蛋白、Ⅰ型胶原蛋白在vps4b基因 敲除鼠磨牙牙胚发育中的时空表达[J]. 华西口腔医学杂志, 2019, 37(3): 248-252. DOI: 10.7518/hxkq.2019.03.004 |
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| 作者姓名： | 陈栋 王莹莹 李晓聪 鲁方丽 李强 |
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| 作者单位： | 郑州大学第一附属医院口腔科,郑州,450000;濮阳市人民医院口腔科,濮阳,457099 |
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| 基金项目： | 国家自然科学基金(81470033) |
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| 摘 要： | 目的 研究vps4b基因突变对牙齿发育相关蛋白——牙本质涎磷蛋白（DSPP）和Ⅰ型胶原蛋白（COL-Ⅰ）表达的影响。方法 取胚胎E13.5 d、E14.5 d、E16.5 d的胎鼠头部及出生后P2.5 d、P7 d的幼鼠下颌骨组织,石蜡包埋后获取第一磨牙牙胚组织切片,采用免疫组织化学染色法检测野生型小鼠和vps4b基因敲除小鼠牙胚中DSPP、COL-Ⅰ的表达。结果 野生鼠蕾状期和帽状期DSPP、COL-Ⅰ均未表达;钟状期DSPP在内釉上皮和牙乳头中有表达,COL-Ⅰ表达于牙乳头和牙囊;分泌期和矿化期DSPP、COL-Ⅰ在成釉细胞、成牙本质细胞、牙囊内均有表达,COL-Ⅰ在牙乳头内亦可见表达。小鼠vps4b基因敲除后,DSPP在钟状期牙乳头和分泌期牙乳头及牙囊内未见表达,COL-Ⅰ在钟状期及矿化期表达部位与野生型小鼠一致,分泌期在牙乳头的表达发生改变。结论 vps4b基因在牙胚发育中发挥重要作用;DSPP、COL-Ⅰ的表达可能受vps4b基因的调控,并与vps4b共同调节牙齿牙本质的发育。
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| 关 键 词： | 牙本质涎磷蛋白 Ⅰ型胶原蛋白 vps4b基因敲除鼠 牙胚发育 |
| 收稿时间： | 2018-12-23 |
| 修稿时间： | 2019-03-19 |
Spatio-temporal expression of dentin sialophosphoprotein and collagen Ⅰ during molar tooth germ development in vps4b knockout mouse |
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| Dong Chen,Yingying Wang,Xiaocong Li,Fangli Lu,Qiang Li. Spatio-temporal expression of dentin sialophosphoprotein and collagen Ⅰ during molar tooth germ development in vps4b knockout mouse[J]. West China journal of stomatology, 2019, 37(3): 248-252. DOI: 10.7518/hxkq.2019.03.004 |
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| Authors: | Dong Chen Yingying Wang Xiaocong Li Fangli Lu Qiang Li |
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| Affiliation: | 1.Dept. of Stomatology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, China2.Dept. of Stomatology, Puyang People’s Hospital, Puyang 457099, China |
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| Abstract: | Objective To verify the effect of the mutant gene vps4b on the expression of tooth development-related proteins, dentin sialophosphoprotein (DSPP) and collagenⅠ (COL-Ⅰ). Methods Paraffin tissue sections of the first molar tooth germ were obtained from the heads of fetal mice at the embryonic stages of 13.5, 14.5, and 16.5 days and from the mandibles of larvae aged 2.5 and 7 days after birth. The immunohistochemical method was used to detect the expression and location of DSPP and COL-Ⅰ in wild-type mouse and vps4b knockout mouse. Results DSPP and COL-Ⅰ were not found in the bud and cap stages of wild-type mouse molar germ. In the bell stage, DSPP was positively expressed in the inner enamel epithelium and dental papilla, whereas COL-Ⅰ was strongly expressed in the dental papilla and dental follicle. During the secretory and mineralized periods, DSPP and COL-Ⅰ were intensely observed in ameloblasts, odontoblasts, and dental follicles, but COL-Ⅰ was also expressed in the dental papilla. After vps4b gene knockout, DSPP was not expressed in the dental papilla of the bell stage and in the dental papilla and dental follicle of the secretory phase. The expression position of COL-Ⅰ in the bell and mineralization phase was consistent with that in the wild-type mice. Moreover, the expression of COL-Ⅰ in the dental papilla changed in the secretory stage. Conclusion Gene vps4b plays a significant role in the development of tooth germ. The expression of DSPP and COL-Ⅰ may be controlled by gene vps4b and regulates the development of tooth dentin and cementum together with vps4b. |
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| Keywords: | dentin sialophosphoprotein collagenⅠ vps4b knockout mouse tooth germ development |
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