| 去甲斑蝥酸钠对肝癌异位移植瘤小鼠肠道微生态的影响初探* |
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| 引用本文： | 裴颖,黄峰,殷宏振,郭化磊. 去甲斑蝥酸钠对肝癌异位移植瘤小鼠肠道微生态的影响初探*[J]. 实用肝脏病杂志, 2019, 22(4): 486-489. DOI: 10.3969/j.issn.1672-5069.2019.04.009 |
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| 作者姓名： | 裴颖 黄峰 殷宏振 郭化磊 |
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| 作者单位： | 712000 陕西省咸阳市陕西中医药大学第一临床医学院 |
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| 基金项目： | * 陕西省自然科学基础研究计划项目（编号：2014JM4175） |
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| 摘 要： | 目的 初步探讨去甲斑蝥酸钠对肝癌异位移植瘤小鼠肠道微生态的影响。方法 将12 只小鼠随机分为去甲斑蝥酸钠注射液和生理盐水处理组及对照组,建立H22 肝癌异位移植瘤小鼠模型。造模成功后正常饲养动物2 w,然后采集小鼠粪便,并进行Illumina miseq 2×300 bp 高通量测序及生物信息学分析。结果 各组样本所包含物种量丰富,且物种丰度和均匀度趋于一致;去甲斑蝥酸钠注射液组与生理盐水处理组或对照组比,存在显著的菌群差异;在门水平上,差异最显著的菌群为变形菌门,去甲斑蝥酸钠注射液组变形菌门所占比例为9.1%,显著高于生理盐水处理组或对照组（分别为2.8%和2.3%,P<0.05）;在属水平上,差异显著的菌群包括拟杆菌属等,生理盐水处理组拟杆菌属所占比例为26.8%,显著高于去甲斑蝥酸钠注射液组或对照组（分别为13.2%和6.8%,P<0.05）。结论 去甲斑蝥酸钠对肝癌异位移植瘤小鼠肠道微生态有显著的影响,或许有助于抗肿瘤作用。
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| 关 键 词： | 肝癌 去甲斑蝥酸钠 肠道微生态 小鼠 |
| 收稿时间： | 2018-09-13 |
Preliminary study on intestinal microecology in mice with xenograft hepatoma intervened by sodiumnorcantharidate |
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| Pei Ying,Huang Feng,Yin Hongzhen,et al. Preliminary study on intestinal microecology in mice with xenograft hepatoma intervened by sodiumnorcantharidate[J]. Journal of Clinical Hepatology, 2019, 22(4): 486-489. DOI: 10.3969/j.issn.1672-5069.2019.04.009 |
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| Authors: | Pei Ying Huang Feng Yin Hongzhen et al |
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| Affiliation: | First Clinical Medical College,Shaanxi University of Traditional Chinese Medicine,Xianyang 712000,Shaanxi Province,China |
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| Abstract: | Objective To explore the changes of intestinal microecology in mice with xenograft hepatoma intervened by sodium norcantharidate.Methods Twelve Kunming mice were randomly divided into sodium nor-cantharidate-intervend,saline-intervend and control groups,and a mouse model of H22 hepatoma xenograft was es-tablished in sodium norcantharidate and saline groups. The mice were normally fed for 2 weeks after the success-ful establishment of the model. Then,the fecal samples were collected and Illumina miseq 2×300 bp high -throughput sequencing and bioinformatics analysis were performed.Results Every samples in each group con-tained abundant species,and species abundance and evenness tended to be consistent；there were significant differ-ences as respect to bacterial communities among sodium norcantharidate,saline and control group；at phylum,the most significantly different bacterial community was Proteobacteria and in sodium norcantharidate-intervend group,the proportion of Proteobacteria was 9.1%,significantly higher than those in saline group or the control group（2.8% and 2.3% ,respectively，P <0.05）;in genus，the different bacterial community included Bacteroides，etc；in saline-intervened group，the proportion of Bacteroides was 26.8%,significantly higher than those in sodium norcan-tharidate group or the control group （13.2% and 6.8%,respectively,P<0.05）. Conclusion There is a obvious change in intestinal microecology in mice with hepatoma xenograft receiving sodium norcantharidate intervention,which might be related to the anti-tumor effect of this agent. |
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| Keywords: | Hepatoma Sodium norcantharidate Intestinal microecology Mice |
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