| 口腔鳞癌中染色体9p杂合性丢失的研究 |
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| 引用本文： | 肖林,IOL Ng,ZC Luo. 口腔鳞癌中染色体9p杂合性丢失的研究[J]. 华西口腔医学杂志, 2001, 19(5): 275-277 |
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| 作者姓名： | 肖林 IOL Ng ZC Luo |
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| 作者单位： | 610041 四川大学华西医院肿瘤中心(肖 林),香港大学玛丽医院(IOLNg, ZC Luo) |
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| 摘 要： | 目的:探讨口腔鳞状细胞癌中9号染色体短臂等位基因的杂合性丢失和微卫星不稳定与口腔鳞状细胞癌发生、发展之间的关系。方法:采用PCR法检测24例口腔鳞状细胞癌中染色体9p上8个微卫星多态位点。结果:在 24例口腔鳞状细胞癌中,10例(41167%)鳞癌组织至少有一个微卫星位点发生杂合性丢失。其主要发生在染色体 9p21的D9S171(21105%)和D9S304(10.00%),以及9p22-23的D9S168(22122%)和D9S162(15138%)。然而,这些基因位点的杂合性丢失与肿瘤病理学类型、肿瘤的大小及转移性在统计学上无显著相关性(P>0105)。此外,微卫星不稳定仅在2例患者中出现,没有1例患者符合微卫星不稳定的判定标准,即两个或两个以上的微卫星多态位点的异常。结论:本研究中发现的口腔鳞状细胞癌在染色体9p21-23区域发生高频率的杂合性丢失,提示在9p21-23区域可能存在多个与部分口腔鳞状细胞癌相关的肿瘤抑制基因,而微卫星不稳定与口腔鳞状细胞癌发生的关系不大。
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| 关 键 词： | 口腔鳞癌 杂合性丢失 染色体9p |
| 收稿时间： | 2001-10-25 |
| 修稿时间： | 2000-05-31 |
Allele-specific Chromosome 9p Deletion in Oral Cancer |
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| IOL Ng,ZC Luo. Allele-specific Chromosome 9p Deletion in Oral Cancer[J]. West China journal of stomatology, 2001, 19(5): 275-277 |
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| Authors: | IOL Ng ZC Luo |
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| Affiliation: | Xiao Lin Cancer Center,the First Affiliated Hospital,West China UniversityofMedicalSciences IOLNg, ZC Luo Queen Mary Hospital,The University of HongKong |
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| Abstract: | Objective:The aim of this study was to investigate the relationship between loss of heterozygosity (LOH) on chromosome 9p and the pathogenesis of oral squamous cell carcinoma (OSCC). Methods: A total of 24 human OSCC specimens were analyzed for LOH on chromosome 9p using 8 microsatellite markers by means of polymerase chain reaction. Results: In 24 cases of OSCC, LOH on chromosome 9p was identified in 10 of 24 cases (41 67%) with at least one marker. The main LOH were found on 9p21 at locus D9S171 (21 05%) and D9S304 (10.00%). There were also a deletion on 9p22_23 at locus D9S168 (22 22%) and D9S162 (15 38%). However, there was no statistically significant correlation between LOH at these loci with such clinical parameters as pathological types, tumor size and lymph_node metastasis. Microsatellite instability (MSI) is rare for any of the 8 markers in 24 OSCC, No MSI could observed using the common criteria for defining MSI_its detection in two or more markers. Conclusion: We found that high frequency of LOH occurred at 9p21_23 band. Their results indicate that more than one tumor suppressor genes at chromosome 9p21_23 region related to a subset of OSCC, while MSI might not be a crucial event.; |
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| Keywords: | oral squamous cell carcinoma loss of heterozygosity microsatellite instability chromosome 9p |
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