首页 | 本学科首页   官方微博 | 高级检索  
     


Characterisation and Expression Analysis of MrLip1, a Class 3 Family Lipase of Malassezia restricta
Authors:Minji Park  Won Hee Jung  Song Hee Han  Young Hoon Lee  Yang Won Lee
Affiliation:1. Department of Systems Biotechnology, Chung‐Ang University, Anseong, Korea;2. Department of Dermatology, School of Medicine, Konkuk University, Seoul, Korea;3. Department of Chemistry, KAIST, Daejeon, Korea;4. Research Institute of Medical Science, Konkuk University, Seoul, Korea
Abstract:The genus Malassezia is associated with a wide range of skin diseases and is the predominant fungal genus isolated from human skin. Of the 14 Malassezia species identified, M. restricta is the most abundant fungal species found from both healthy and diseased skin. Emerging evidences have suggested that extracellular lipases of Malassezia play a critical role in its survival on the host skin surface. This study aimed to characterise the lipase 1 homologue (MrLip1) in M. restricta and to analyse its expression under different environmental conditions. The full sequence of the gene encoding MrLip1 was determined by rapid amplification of cDNA ends, and it was then heterologously expressed in Pichia pastoris. MrLip1 protein was successfully purified and used for lipase assay and specific antibody generation for use in expression analysis. The optimum pH and temperature for the activity of purified MrLip1 were pH 5.0 and 34 °C respectively. Furthermore, the expression of MrLip1 peaked at a similar pH and temperature, suggesting that the optimal conditions for MrLip1 protein activity and expression are similar to that found on the human skin surface. This study provides data to improve our understanding of the role and characteristics of lipase 1 in M. restricta.
Keywords:   Malassezia restricta     lipase  skin     pH     temperature
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号