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| 组蛋白乙酰化修饰调控流感病毒复制中间体dsRNA引起的IL-6激活表达 | |
| 引用本文： | 汤必奎,吴守伟,胡明洁,刘小粉. 组蛋白乙酰化修饰调控流感病毒复制中间体dsRNA引起的IL-6激活表达[J]. 南方医科大学学报, 2013, 33(2): 282-286 |
| 作者姓名： | 汤必奎 吴守伟 胡明洁 刘小粉 |
| 作者单位： | 蚌埠医学院生物科学系,安徽蚌埠,233030 |
| 基金项目： | 安徽省自然科学基金，安徽省高等学校优秀青年人才基金 |
| 摘 要： | 目的研究组蛋白乙酰化修饰在流感病毒复制中间体dsRNA引起IL-6 表达中的调控作用。方法以病毒复制中间体 dsRNA为诱导物，利用组蛋白去乙酰化酶（HDAC）抑制剂、DNA甲基转移酶（DNMT）抑制剂或HDAC siRNA分别处理A549细 胞，双荧光素酶报告系统分析IL-6启动子活性、RT-PCR检测mRNA转录水平以及ELISA检测蛋白表达。结果dsRNA可激活 IL-6启动子活性、增强mRNA转录水平和蛋白的分泌表达，用HDAC抑制剂TSA处理或HADC siRNA干扰均可显著增强IL-6 启动子活性和mRNA转录，同时，HDAC抑制剂TSA和DNA甲基转移酶抑制剂DAC在调控IL-6表达过程中无协同作用。结 论在流感病毒复制中间体dsRNA引起IL-6表达上调过程中受到组蛋白乙酰化修饰调控。 |
| 关 键 词： | 组蛋白乙酰化 白细胞介素-6 流感病毒复制中间体 dsRNA |
Histone acetylation modulates influenza virus replicative intermediate dsRNA-induced interleukin-6 expression in A549 cells |
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| TANG Bikui , WU Shouwei , HU Mingjie , LIU Xiaofen. Histone acetylation modulates influenza virus replicative intermediate dsRNA-induced interleukin-6 expression in A549 cells[J]. Journal of Southern Medical University, 2013, 33(2): 282-286 | |
| Authors: | TANG Bikui WU Shouwei HU Mingjie LIU Xiaofen |
| Affiliation: | Department of Life Science,Bengbu Medical College,Bengbu 233030,China |
| Abstract: | Objective To investigate the role of histone acetylation in regulating influenza virus replicative intermediate double-stranded RNA (dsRNA)-induced interleukin-6 (IL-6) expression in A549 cells. Methods A549 cells were treated with influenza virus replicative intermediate dsRNA, histone deacetylase (HDAC) inhibitor trichostatin A (TSA), or HADC small interfering RNA (siRNA). The changes in the cellular IL-6 promoter activities were detected by dual-luciferase assay, and IL-6 mRNA and protein expressions in the cells were determined using real-time RT-PCR and ELISA, respectively. Results Influenza virus replicative intermediate dsRNA obviously up-regulated IL-6 expression in the cells. HDAC inhibitor TSA significantly enhanced the activity of IL-6 promoter and increased IL-6 mRNA expression in A549 cells, and HDAC3 may play an important role in this process. HDAC inhibitor TSA and DNMT inhibitor DAC showed no synergic effect in regulating IL-6 expression. Conclusion Influenza virus replicative intermediate dsRNA-induced IL-6 expression in A549 cells is regulated by histone acetylation. |
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