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| 槲皮素对胶质瘤U87细胞侵袭、迁移、增殖及其细胞周期的影响 | |
| 引用本文： | 苑召虎,胡子有,张兰兰,颜晓慧,王惠丽,吴炳义. 槲皮素对胶质瘤U87细胞侵袭、迁移、增殖及其细胞周期的影响[J]. 南方医科大学学报, 2013, 33(2): 207-211 |
| 作者姓名： | 苑召虎 胡子有 张兰兰 颜晓慧 王惠丽 吴炳义 |
| 作者单位： | 南方医科大学南方医院临床医学实验研究中心,广东广州,510515 |
| 摘 要： | 目的探讨黄酮类化合物槲皮素对人胶质瘤U87细胞侵袭、迁移、增殖及其细胞周期的影响。方法将U87细胞分成Q0、 Q50、Q100和Q150 4个组，Q0组加适量二甲亚砜（槲皮素溶剂）处理，Q50、Q100和Q150组槲皮素的浓度分别为50、100、150 μmol/L。分别 通过transwell体外侵袭实验、transwell体外迁移实验、Click-iT Edu test技术和流式细胞术检测槲皮素对其侵袭迁移能力，增殖 能力及其细胞周期的影响。结果槲皮素处理36 h后，与对照组相比，Q50、Q100和Q150组的侵袭细胞比率分别为：52.08%、24.63% 和13.13%，P均<0.05。槲皮素处理12 h后，与对照组相比，Q50、Q100和Q150的迁移细胞比率分别为：49.46%、26.78%和14.56%，P 均<0.05。槲皮素处理24 h后，Q0、Q50、Q100和Q150组的增殖细胞百分比分别为25.21%、18.38%、16.74%和15.24%。G0/Gl期C6细 胞所占比例分别为71.14%、72.71%、69.29%、66.47%，S 期分别为25.32%、22.48%、21.96%、23.32%，G2/M 期分别为3.53%、 4.80%、8.75%、10.25%；Q100、Q150组的G2/M期细胞比例与Q0组比较，P均<0.05。结论槲皮素能明显降低胶质瘤细胞的侵袭迁移 能力；并可通过阻断U87细胞的细胞周期进程来抑制其增殖。 |
| 关 键 词： | 槲皮素 U87胶质瘤细胞 侵袭 迁移 增殖 细胞周期 |
Effect of quercetin on invasion, migration, proliferation and cell cycle of glioma U87 cells |
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| YUAN Zhaohu , HU Ziyou , ZHANG Lanlan , YAN Xiaohui , WANG Huili , WU Bingyi. Effect of quercetin on invasion, migration, proliferation and cell cycle of glioma U87 cells[J]. Journal of Southern Medical University, 2013, 33(2): 207-211 | |
| Authors: | YUAN Zhaohu HU Ziyou ZHANG Lanlan YAN Xiaohui WANG Huili WU Bingyi |
| Affiliation: | Research Center of Clinical Medicine,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China |
| Abstract: | Objective To explore the effect of quercetin on the invasion, migration, proliferation and cell cycle of glioma U87 cells. Methods Glioma U87 cells were treated with 50, 100, or 150 μmol/L quercetin (Q50, Q100 and Q150 groups, respectively) or with DMSO (Q0 group). Transwell in vitro invasion and migration assays, Click-iT Edu test and flow cytometry were performed to evaluate the effect of quercetin on the invasion, migration, proliferation and cell cycle of U87 cells. Results After 36 h of quercetin treatment, the cells in Q50, Q100 and Q150 groups showed invasive cell percentages (relative to Q0 group) of 52.08%, 24.63%, and 13.13%, respectively (P<0.05). After quercetin treatment for 12 h, the migrating cell percentages (relative to Q0 group) in Q50, Q100 and Q150 groups were 49.46%, 26.78%, and 14.56%, respectively (P<0.05). After 24 h of quercetin treatment, the cell proliferation ratios in Q0, Q50, Q100 and Q150 groups were 25.21%, 18.38%, 16.74% and 15.24%; the cell percentages in phase G0/Gl were 71.14%, 72.71%, 69.29%, and 66.47%, phase S were 25.32%, 22.48%, 21.96%, and 23.32%, and phase G2/M were 3.53%, 4.80%, 8.75%, and 10.25% in the 4 groups, respectively, showing a significant difference between groups Q100, Q150 and group Q0 in phase G2/M cell percentages (P<0.05). Conclusion Quercetin can significantly inhibit the invasion, migration and proliferation of glioma U87 cells by blocking the cell cycle progression. |
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