Detection of Legionella pneumophila using a real-time PCR hybridization assay |
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| Authors: | Ballard A L Fry N K Chan L Surman S B Lee J V Harrison T G Towner K J |
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| Affiliation: | Department of Microbiology and PHLS Laboratory, University Hospital, Nottingham NG7 2UH, United Kingdom. |
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| Abstract: | A real-time PCR hybridization assay for Legionella pneumophila is described; the assay uses LightCycler (Idaho Technology) methodology to specifically detect 2.5 CFU/reaction, equivalent to 1,000 CFU/liter of starting water sample. The assay, including DNA extraction and confirmation of product identity, is completed within 90 min of receipt of a sample. |
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