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| 小鼠胚胎干细胞分泌因子对前列腺癌细胞作用的体外研究 | |
| 引用本文： | 翟红运,张登禄,王光杰,孔峰,程广辉,赵升田. 小鼠胚胎干细胞分泌因子对前列腺癌细胞作用的体外研究[J]. 山东大学学报(医学版), 2015, 53(9): 8-12. DOI: 10.6040/j.issn.1671-7554.0.2015.219 |
| 作者姓名： | 翟红运 张登禄 王光杰 孔峰 程广辉 赵升田 |
| 作者单位： | 1. 山东大学第二医院 泌尿外科, 山东 济南 250033;2. 山东大学第二医院 中心实验室, 山东 济南 250033 |
| 基金项目： | 国家自然科学基金(81272815);泰山学者特聘教授科研基金 |
| 摘 要： | 目的 研究小鼠胚胎干细胞(ESC)分泌因子对前列腺癌细胞RM-1生物学特性的影响,并探讨其作用机制。方法 采用Transwell小室在体外建立ESC联合小鼠胚胎成纤维细胞(MEF)与RM-1细胞的非接触式共培养体系作为实验干预(实验组),同时建立MEF与RM-1细胞的共培养体系作为阴性对照(对照组)。共培养72 h后,检测实验组与对照组RM-1细胞的增殖水平,比较两组细胞的形态学、细胞周期、凋亡水平、迁移与侵袭能力的差异。结果 与对照组比较,实验组RM-1细胞的形态表现为排布稀疏,分裂减少,死亡增多,部分细胞呈现凋亡迹象;实验组RM-1细胞增殖减慢(P<0.01),细胞周期存在G1→S期阻滞且凋亡增加,以晚期凋亡为主(P均<0.01);实验组RM-1细胞的迁移和侵袭能力减弱(P均<0.05)。结论 ESC分泌因子可通过阻滞RM-1细胞周期、促进其凋亡使RM-1细胞的增殖减慢,同时减弱其迁移与侵袭能力而发挥抗肿瘤作用。 |
| 关 键 词： | 胚胎干细胞 分泌因子 前列腺癌细胞RM-1 肿瘤 细胞环境 小鼠 |
| 收稿时间： | 2015-02-28 |
Effects of factors secreted by mouse embryonic stem cells on prostate cancer cells in vitro |
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| ZHAI Hongyun,ZHANG Denglu,WANG Guangjie,KONG Feng,CHENG Guanghui,ZHAO Shengtian. Effects of factors secreted by mouse embryonic stem cells on prostate cancer cells in vitro[J]. Journal of Shandong University:Health Sciences, 2015, 53(9): 8-12. DOI: 10.6040/j.issn.1671-7554.0.2015.219 | |
| Authors: | ZHAI Hongyun ZHANG Denglu WANG Guangjie KONG Feng CHENG Guanghui ZHAO Shengtian |
| Affiliation: | 1. Department of Urology, Jinan 250033, Shandong, China;2. Central Laboratory, Second Hospital of Shandong University, Jinan 250033, Shandong, China |
| Abstract: | Objective To study the effects of factors secreted by mouse embryonic stem cells (ESC) on the biological characteristics of prostate cancer cells RM-1 and to explore the mechanism. Methods A non-contact co-culture system was established with Transwell chambers in vitro. The chambers connecting ESC combined with mouse embryonic fibroblasts (MEF) and RM-1 cells served as experimental group, while the chambers connecting MEF and RM-1 cells were negative controls. After cells were co-cultured for 72 h, the proliferation of RM-1 cells in the two groups were measured. Differences of RM-1 cells in cell morphology, cell cycle, apoptotic level, migration and invasion ability between the two groups were observed. Results The RM-1 cells in experimental group were loosely arranged with less cell division and more cell death, and some cells showed signs of apoptosis. The RM-1 cells in experimental group proliferated more slowly (P<0.01), with cell cycle arrest between phase G1 and S in addition with increased apoptosis, especially late stage apoptosis (both P<0.01). The RM-1 cells in experimental group showed weaker migration and invasion ability (both P<0.05). Conclusion The factors secreted by ESC exhibited anti-tumor effects. They inhibited RM-1 cell proliferation by arresting cell cycle, increasing cell apoptosis, and weakening cell migration and invasion ability. |
| Keywords: | Secreted factors Prostate cancer cell RM-1 Cellular environment Embryonic stem cell Mouse Neoplasia |
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