| Abstract: | Investigation intracellular trafficking of siRNAs following their delivery to cells is of great interest to elucidate dynamics of siRNA in cytoplasm. In this study, we present a novel confocal laser scanning microscopy (CLSM) method to evaluate a novel delivery system of 3′-peptide-siRNA therapeutic, which was named 3′-pAs-siRNA/CLD. This method could not only calculate the content of the intracellular 3′-peptide-siRNA, but also quantify its co-localization with cellular substructure. We observed that 3′-pAs-siRNA/CLD, which provided the better antitumor capability, also had a better cell uptake, endosome escape and a longer retention time in A375. This novel strategy was proved to be efficient, quantified and visualized, thus making the dynamics research of siRNA in cytoplasm clear and simplified. |
