| 尼古丁抑制成牙本质细胞增殖及作用机制的研究 |
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| 引用本文： | 吴礼安,文玲英,杨富生,王小竞. 尼古丁抑制成牙本质细胞增殖及作用机制的研究[J]. 华西口腔医学杂志, 2008, 26(2): 186-188 |
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| 作者姓名： | 吴礼安 文玲英 杨富生 王小竞 |
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| 作者单位： | 第四军医大学口腔医院,儿童口腔科,陕西,西安,710032;第四军医大学口腔医院,儿童口腔科,陕西,西安,710032;第四军医大学口腔医院,儿童口腔科,陕西,西安,710032;第四军医大学口腔医院,儿童口腔科,陕西,西安,710032 |
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| 基金项目： | 第四军医大学校科研和教改项目 |
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| 摘 要： | 目的观察尼古丁对成牙本质细胞增殖的抑制作用，并检测其对成牙本质细胞中Ca2+浓度的影响，以探讨尼古丁抑制成牙本质细胞的分子机制。方法体外培养成牙本质细胞系MDPC- 23细胞, 按每毫升2×104个接种，随机分为实验组和对照组，对照组不加任何刺激，实验组施加质量浓度为100 μg/mL尼古丁，并于8 h后加入浓度为10 μmol/L BrdU进行细胞周期标记，刺激24 h后固定细胞，行免疫荧光抗BrdU染色，碘化丙锭（PI）复染胞核，荧光显微镜下计数细胞总数与BrdU阳性细胞数，计算S期阳性细胞率并进行统计学分析。培养成牙本质细胞于特制培养皿中，施加质量浓度为100 μg/mL尼古丁刺激，激光共聚焦显微镜下检测成牙本质细胞中Ca2+浓度的动态变化。结果实验组、对照组S期阳性细胞率分别为36.3%、48.2%，实验组显著低于对照组。尼古丁刺激后，成牙本质细胞中Ca2+浓度迅速升高，在较高水平维持一段时间后缓慢下降。结论尼古丁可抑制成牙本质细胞增殖，这种作用同尼古丁升高成牙本质细胞中Ca2+浓度有关。
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| 关 键 词： | 尼古丁 成牙本质细胞 增殖 |
| 文章编号： | 1000-1182(2008)02-0186-03 |
| 收稿时间： | 2008-04-25 |
| 修稿时间： | 2007-07-11 |
Effects and molecular mechanism of nicotine on odontoblasts |
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| WU Lian,WEN Ling-ying,YANG Fu-sheng,WANG Xiao-jing. Effects and molecular mechanism of nicotine on odontoblasts[J]. West China journal of stomatology, 2008, 26(2): 186-188 |
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| Authors: | WU Lian WEN Ling-ying YANG Fu-sheng WANG Xiao-jing |
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| Affiliation: | Dept. of Pediatric Dentistry, College of Stomatology, The Fourth Military Medical University, Xi′an 710032, China |
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| Abstract: | OBJECTIVE: To observe the effects of nicotine on the proliferation of odontoblasts and explore the possible mechanism. METHODS: Odontoblasts MDPC-23 were cultured, inoculated and divided into two groups randomly. With no stimuli added for the control group, the experimental group was stimulated by 100 microg/mL nicotine. After 8 hours, 10 micromol/L BrdU was added to label cells at S stage in cell cycle. 24 hours later, odontoblasts were fixed and immunofluorescence staining was performed with specific mouse BrdU antibody. After counterstaining with propidium iodide, BrdU positive cells were arbitrarily scored microscopically by an independent estimation conducted three times, and the corresponding total cell number in the same vision were counted in both groups. BrdU positive cell rates were calculated and compared statistically. At the same time, odontoblasts MDPC-23 were cultured and stimulated by 100 microg/mL nicotine, the dynamic Ca2+ concentration inside the cytoplasm were detected immediately by a confocal laser scanning microscope. RESULTS: The ratio of S stage cells in the experimental group was 36.3% significantly lower than that (48.2%) in the control group. After the addition of 100 microg/mL nicotine, the Ca2+ concentration inside the cytoplasm rose rapidly, sustained at a high level for a short time and then relapsed gradually. CONCLUSION: Nicotine had inhibitory effects on the proliferation of odontoblasts MDPC-23, which might be related to the increased Ca2+ concentration in the cytoplasm. |
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| Keywords: | nicotine odontoblast proliferation |
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