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| 伯氏疟原虫感染不同年龄小鼠免疫反应特点 | |
| 引用本文： | 单颖, 刘军, 潘艳艳, 李莹, 曹雅明. 伯氏疟原虫感染不同年龄小鼠免疫反应特点[J]. 中国公共卫生, 2013, 29(9): 1289-1291. DOI: 10.11847/zgggws2013-29-09-13 |
| 作者姓名： | 单颖 刘军 潘艳艳 李莹 曹雅明 |
| 作者单位： | 1.辽宁医学院免疫教研室, 辽宁 锦州 121001;2.中国医科大学免疫教研室 |
| 摘 要： | 目的 了解伯氏疟原虫(Plasmodium berghei NK65,P.b NK65)感染幼年和中年C57BL/6小鼠免疫反应特点。方法 P.b NK65感染3周龄幼年和8月龄中年C57BL/6小鼠,采用流式细胞分析技术检测不同年龄组小鼠脾细胞悬液中髓样树突状细胞(dendritic cells,DCs)数量,表面表达MHCⅡ类分子和CD86分子的DCs数量以及分泌IgG抗体的B细胞数量;ELISA方法检测脾细胞培养上清中IFN-γ和IL-4水平。结果 3周小鼠DCs数量[(40.08±3.54)×105]在感染后5 d明显升高,8月龄鼠在感染后5 d[(103.47±11.07)×105]及8 d[(104.04±1.92)×105]明显升高;3周鼠表面表达MHCⅡ类分子和CD86分子的DCs数量在感染后明显升高,8月龄鼠在感染后8 d表达MHCⅡ类分子[(239.40±16.32)×105]和CD86分子[(148.48±14.84)×105]数量明显高于3周鼠(P<0.05);3周鼠[(251.01±34.02) pg/mL]和8月龄鼠[(584.17±54.03) pg/mL] IFN-γ水平在感染后5 d均明显升高,2组比较差异有统计学意义(P<0.05)。结论 3周和8月龄C57BL/6小鼠感染P.b NK65后,其细胞免疫和体液免疫存在明显差异。 |
| 关 键 词： | 伯氏疟原虫 不同年龄 免疫反应 |
| 收稿时间： | 2013-04-24 |
Characterization of immune response to infection of Plasmodium berghei NK65 in mice of different age |
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| SHAN Ying, LIU Jun, PAN Yan-yan.et al, . Characterization of immune response to infection of Plasmodium berghei NK65 in mice of different age[J]. Chinese Journal of Public Health, 2013, 29(9): 1289-1291. DOI: 10.11847/zgggws2013-29-09-13 | |
| Authors: | SHAN Ying LIU Jun PAN Yan-yan.et al |
| Affiliation: | 1.Department of Immunology, Liaoning Medical College, Jinzhou, Liaoning Province 121001, China |
| Abstract: | ObjectiveTo investigate the characteristics of immune response to infection of Plasmodium berghei NK65(P.b NK65)in young and middle aged C57BL/6 mice.MethodsThree weeks and 8 months aged C57BL/6 mice were infected by P.b NK65 parasitized erythrocytes.Flow cytometry was applied to quantitatively analyze myeloid dendritic cells(DCs)and DCs expressing major histocompatibility complex classⅡ (MHCⅡ)or CD86.The levels of interferon gamma(IFN-γ)and interleukin-4(IL-4)in spleen cell supermatants were dynamically detected by enzyme-linked immunosorbent assay(ELISA).ResultsThe number of myeloid DCs(40.08×105)in young aged mice significantly increased 5 days after the infection.The number of myeloid DCs 5 and 8 days after the infection was 103.47×105 and 104.04×105 in the middle aged mice.The numbers of DCs expressing MHCⅡ or CD86 were 50.16×105 and 45.39×105 in young aged mice,and in middle aged mice the numbers were 239.40×105 and 148.48×105,which was markedly more than young aged mice.The level of IFN-γ significantly increased both in young aged(251.01 pg/ml)and middle aged mice(584.17 pg/ml).ConclusionThe cellular and humoral immune responses to infection of P.b NK65 are different between the young and middle aged C57BL/6 mice. |
| Keywords: | Plasmodium berghei different age immune response |
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