| 血管紧张素Ⅱ对大鼠肾入球小动脉细胞内钙离子浓度的影响及Losartan的拮抗作用 |
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| 引用本文： | 孙洞箫, 周盾, 邱振宇. 血管紧张素Ⅱ对大鼠肾入球小动脉细胞内钙离子浓度的影响及Losartan的拮抗作用[J]. 中国现代应用药学, 2010, 27(10): 857-860. |
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| 作者姓名： | 孙洞箫 周盾 邱振宇 |
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| 作者单位： | 1.辽宁医学院附属第一医院肾内科，辽宁 锦州 121001 |
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| 基金项目： | 辽宁省教育厅2008年资助项目（2008407） |
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| 摘 要： | 目的 探讨血管紧张素Ⅱ(AngⅡ)对肾小球入球小动脉细胞内钙离子浓度([Ca2+]i)的影响与高血压肾小动脉重建的关系及Losartan的治疗作用。方法 细胞培养：大鼠肾小球入球小动脉细胞随机分为4组。对照组：不加AngⅡ处理；AngⅡ组：加入终浓度为0.1 μmol·L-1的AngⅡ；Losartan组：加入终浓度为50 μmol·L-1的Losartan；AngⅡ+Losartan组：同时加入Losartan 50 μmol·L-1和AngⅡ0.1 μmol·L-1。负载后上机检测[Ca2+]i。结果 细胞培养显示AngⅡ引起了细胞收缩变化，表现为胞突变细变短，胞突回缩，胞体变圆，细胞长度与直径均明显缩小。Losartan处理组较AngⅡ组细胞形态变化减轻且Losartan对AngⅡ增高RASMCs内[Ca2+]i有明显抑制作用。结论 AngⅡ可引起肾小球入球小动脉细胞内[Ca2+]i的上升，导致细胞收缩，因此Ca2+超载可能是肾素-血管紧张素系统起作用的重要环节。Losartan可抑制细胞内Ca2+超载。
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| 关 键 词： | 肾小球入球小动脉细胞 高血压 血管紧张素Ⅱ Losartan 细胞内钙离子浓度 |
Effect of Angiotensin II on Intracellular Free Calcium Concentration in RASMCs and Antagonism of Losartan |
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| SUN Dongxiao, ZHOU Dun, QIU Zhenyu. Effect of Angiotensin II on Intracellular Free Calcium Concentration in RASMCs and Antagonism of Losartan[J]. Chinese Journal of Modern Applied Pharmacy, 2010, 27(10): 857-860. |
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| Authors: | SUN Dongxiao ZHOU Dun QIU Zhenyu |
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| Affiliation: | 1.Department of Nephorology, the First Hospital Affiliated Liaoning Medical College, Jinzhou 121001, China |
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| Abstract: | OBJECTIVE To explore the effects of angiotensin（Ang Ⅱ） on intracellular free calcium concentration（[Ca^2＋]i） in RASMCs and antagonism of Losartan.METHODS RASMCs were isolated and divided into four groups： control group,Ang Ⅱ group,Losartan group and Ang Ⅱ ＋Losartan group.Confocal microscope was used with Fluo-3/AM as an indicator to detect the changes of [Ca^2＋]i.RESULTS Compared with control group,intracellular fluorescent intensity of RASMCs had no change in Losartan group,and was significantly higher in Ang Ⅱ group（P〈0.05）.Value in Ang Ⅱ ＋Losartan group was significantly lower than that in Ang Ⅱ group（P〈0.05）.CONCLUSION Ang Ⅱ may induce intracellular calcium overload in RASMCs,which can be significantly reduced by Losartan. |
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| Keywords: | RASMCs hypertension angiotensin Ⅱ Losartan intracellular free calcium concentration |
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