| 靶向抑制miR-19a提高人宫颈癌HeLa细胞对顺铂敏感性的机制研究 |
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| 引用本文： | 崔立春,杜建飞,耿会生,韩良辅,刘金鹏. 靶向抑制miR-19a提高人宫颈癌HeLa细胞对顺铂敏感性的机制研究[J]. 现代肿瘤医学, 2015, 0(5): 604-607. DOI: 10.3969/j.issn.1672-4992.2015.05.08 |
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| 作者姓名： | 崔立春 杜建飞 耿会生 韩良辅 刘金鹏 |
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| 作者单位： | 长安医院肿瘤中心，陕西 西安 710018 |
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| 摘 要： | 目的:研究miR-19a反义寡核苷酸(antisense oligonucleotide，ASO)对人宫颈癌HeLa细胞对顺铂耐药性的影响，并进一步探讨其机制。方法:利用Lipofectamine 2000将 miR-19a-ASO瞬时转染HeLa细胞，实时定量PCR法检测miR-19a表达水平。MTT法检测顺铂对HeLa细胞存活率的影响。Western blot检测PTEN、AKT和p-AKT（Ser473）蛋白表达的变化。结果:miR-19a-ASO明显抑制HeLa细胞miR-19a的表达。MTT结果表明靶向抑制miR-19a能明显促进HeLa细胞对顺铂的敏感性。Western blot结果提示，与对照组比较，干扰miR-19a的HeLa细胞，PTEN表达水平升高，p-AKT（Ser473）表达水平降低。结论:沉默miR-19a可能通过抑制AKT通路提高宫颈癌细胞对顺铂的敏感性，从而为宫颈癌的治疗提供新靶点。
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| 关 键 词： | 宫颈癌 化疗耐药性 miR-19a PTEN AKT通路 |
Targeted suppression of miR - 19a enhanced chemosensitivity of cervical cancer HeLa cells to cisplatin |
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| Cui Lichun,Du Jianfei,Geng Huisheng,Han Liangfu,Liu Jinpeng. Targeted suppression of miR - 19a enhanced chemosensitivity of cervical cancer HeLa cells to cisplatin[J]. Journal of Modern Oncology, 2015, 0(5): 604-607. DOI: 10.3969/j.issn.1672-4992.2015.05.08 |
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| Authors: | Cui Lichun Du Jianfei Geng Huisheng Han Liangfu Liu Jinpeng |
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| Affiliation: | Cancer Center，Chang'an Hospital，Shaanxi Xi'an 710018,China. |
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| Abstract: | Objective:To investigate the effect of miR-19a antisense oligonucleotide （miR-19a-ASO） on cisplatin chemosensitivity in human HeLa cells and its underlying mechanism.Methods:miR-19a-ASO was transfected to HeLa cells using Lipofectamine 2000.Real-time PCR was used to evaluate the miR-19a expression.Cell viability was measured by methylthiazolyltetrazolium （MTT） assay.The expression of PTEN，AKT，and p-AKT(Ser473） protein was detected by Western blot.Results:miR-19a-ASO inhibited the expression of miR-19a effectively.HeLa cells transfected with miR-19a-ASO was sensitized to cisplatin （P<0.05） by MTT assay.Targeting to miR-19a could up-regulate PTEN，and down-regulate p-AKT（Ser473） expression.Conclusion:Silence of miR-19a increase cell sensitivity to cisplatin probably by suppressing AKT signaling pathway，providing a new target for cervical cancer therapy. |
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| Keywords: | cervical cancer chemosensitivity miR-19a PTEN AKT signaling pathway |
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