| 姜黄素对人结肠癌细胞PI3-K/Akt和MEK/ERK信号通路的影响 |
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| 引用本文： | 金 子1,' target='_blank'>2,蔡 颖1,张 晔1,刘云鹏1,曲秀娟1,徐 玲1,侯科佐1. 姜黄素对人结肠癌细胞PI3-K/Akt和MEK/ERK信号通路的影响[J]. 现代肿瘤医学, 2015, 0(12): 1629-1631. DOI: 10.3969/j.issn.1672-4992.2015.12.01 |
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| 作者姓名： | 金 子1 ' target='_blank'>2 蔡 颖1 张 晔1 刘云鹏1 曲秀娟1 徐 玲1 侯科佐1 |
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| 作者单位： | 1.中国医科大学附属第一医院肿瘤内科，辽宁 沈阳 110001;2.沈阳市第五人民医院肿瘤内一科，辽宁 沈阳 110023 |
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| 基金项目： | 国家自然科学基金项目（编号:81270036,30901736）；辽宁省自然科学基金项目（编号:2013021057,2014021069)；辽宁省教育厅优秀人才支持计划项目（编号:LR2014023） |
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| 摘 要： | 目的:探讨姜黄素对人结肠癌RKO细胞PI3-K/Akt和MEK/ERK通路的影响。方法:MTT法检测细胞活力，Western blot检测p-Akt、Akt、p-ERK，ERK及凋亡相关蛋白Bcl-2、Bax的表达。结果:姜黄素作用人结肠癌RKO细胞，24h和48h的IC50值分别为51.69μg/ml和36.12μg/ml。选用50μg/ml的姜黄素分别作用24h和48h，RKO细胞凋亡百分比为26.79%和42.16%，与对照组相比有显著差异(P＜0.05)。进一步检测发现姜黄素（50μg/ml）显著下调了p-Akt 和p-ERK的表达，同时Bcl-2与Bax的比值显著下调。结论:姜黄素可能通过抑制PI3-K/Akt和MEK/ERK信号通路的活化、下调Bcl-2与Bax的比值，从而抑制RKO细胞增殖和诱导细胞凋亡。
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| 关 键 词： | 结肠癌 姜黄素 PI3-K/Akt MEK/ERK 凋亡 |
The effect of curcumin on PI3-K/Akt and MEK/ERK pathway in colon cancer |
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| Jin Zi1,' target='_blank'>2,Cai Ying1,Zhang Ye1,Liu Yunpeng1,Qu Xiujuan1,Xu Ling1,Hou Kezuo1. The effect of curcumin on PI3-K/Akt and MEK/ERK pathway in colon cancer[J]. Journal of Modern Oncology, 2015, 0(12): 1629-1631. DOI: 10.3969/j.issn.1672-4992.2015.12.01 |
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| Authors: | Jin Zi1 ' target='_blank'>2 Cai Ying1 Zhang Ye1 Liu Yunpeng1 Qu Xiujuan1 Xu Ling1 Hou Kezuo1 |
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| Affiliation: | 1.Department of Medical Oncology,The First Hospital of China Medical University,Liaoning Shenyang 110001,China;2.Department of Medical Oncology,The Fifth Hospital of Shenyang,Liaoning Shenyang 110023,China. |
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| Abstract: | Objective:To investigate the effects of curcumin on the PI3-K/Akt and MEK/ERK patheway in human colon cancer.Methods:Cell proliferation was measured using MTT assay.The expressions of p-Akt,Akt,p-ERK,ERK and apoptosis-related proteins were detected by Western blot.Results:Curcumin inhibited RKO cell viability and the concertration of inhibited cell viability (IC50) for 24h and 48h were 51.69μg/ml and 36.12μg/ml.After treatment with 50μg/ml curcumin for 24h and 48h,the rates of cell apoptosis were 26.79% and 42.16%,respectively.And after treated 24h with 50μg/ml curcumin,the expressions of p-Akt and p-ERK were significantly down-regulated in RKO cells and the ratio of Bcl-2/Bax expressions was decreased.Conclusion:Curcumin inhibited the activation of the PI3-K/Akt and MEK/ERK signaling pathway,which consequently down-regulated the value of Bcl-2/Bax ratio in RKO cells. |
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| Keywords: | colon cancer curcumin PI3-K/Akt MEK/ERK apoptosis |
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