| 碘及TSH对甲状腺乳头状癌细胞中miRNA表达的影响 |
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| 引用本文： | 金明月,关海霞,单忠艳. 碘及TSH对甲状腺乳头状癌细胞中miRNA表达的影响[J]. 现代肿瘤医学, 2015, 0(9): 1184-1187. DOI: 10.3969/j.issn.1672-4992.2015.09.03 |
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| 作者姓名： | 金明月 关海霞 单忠艳 |
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| 作者单位： | 中国医科大学附属第一医院内分泌科，内分泌研究所，辽宁 沈阳 110001 |
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| 摘 要： | 目的:体外实验研究人体甲状腺乳头状癌(PTC)细胞中miRNA在碘、TSH影响下的表达变化。方法:体外培养PTC细胞，Western blot法半定量分析p27kip1、AKT、AP-1的表达，RT-PCR法定量分析miRNA-21、miRNA-221、miRNA-222的表达。结果:经不同浓度碘和TSH分别处理PTC细胞，3种miRNA的表达量较对照组均上调；所对应的蛋白p27kip1表达均减少，AP-1及AKT表达较对照组均增加，增加程度与处理因素的浓度未见明显关联。结论:碘、TSH对甲状腺乳头状癌细胞中PI3K/AKT信号通路起到上调作用，从而对甲状腺乳头状癌的发展起到了一定促进作用，且其作用程度与处理浓度无关。
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| 关 键 词： | 甲状腺乳头状癌 miRNA 碘 TSH |
The effect of iodine and TSH on the expression of miRNA in papillary thyroid carcinoma cells |
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| Jin Mingyue,Guan Haixia,Shan Zhongyan. The effect of iodine and TSH on the expression of miRNA in papillary thyroid carcinoma cells[J]. Journal of Modern Oncology, 2015, 0(9): 1184-1187. DOI: 10.3969/j.issn.1672-4992.2015.09.03 |
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| Authors: | Jin Mingyue Guan Haixia Shan Zhongyan |
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| Affiliation: | Department of Endocrinology，Institute of Endocrinology,the First Affiliated Hospital，China Medical University，Liaoning Shenyang 110001,China. |
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| Abstract: | Objective:To investigate the expression change of miRNAs under the treatment of iodine and TSH by means of human thyroid papillary carcinoma (PTC) cells culture in vitro.Methods:Cells were cultured for semi-quantitative analysis of p27kip1，AKT，AP-1 expression by Western blot and quantitative analysis of the three miRNAs' expression by RT-PCR.Results:After treated thyroid papillary carcinoma TPC-1 with different concentrations of TSH and iodide separately，miRNA-21，miRNA-221 and miRNA-222 were all up regulated.The miRNAs corresponding protein p27kip1 expression was reduced.Conversely，AP-1 and AKT were increased.All the changes of the expression of the miRNAs and proteins showed no association with concentration of processing factors.Conclusion:Iodine，TSH can increase PI3K/Akt signaling pathway expression in PTC cells，but no significant difference were observed in the expression levels with different concentrations.Both of them may played a catalytic role in the development of papillary thyroid carcinoma. |
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| Keywords: | papillary thyroid carcinoma miRNA iodine TSH |
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