| 不同试剂脱除猪胸主动脉壁细胞的对比研究 |
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| 引用本文： | 顾春虎,刘维永,张近宝,王云雅,刘洋,谭红梅,宿学家. 不同试剂脱除猪胸主动脉壁细胞的对比研究[J]. 心脏杂志, 2005, 17(3): 207-209. DOI: 10.13191/j.chj.2005.03.10.guchh.004 |
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| 作者姓名： | 顾春虎 刘维永 张近宝 王云雅 刘洋 谭红梅 宿学家 |
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| 作者单位： | 第四军医大学西京医院心脏外科,陕西,西安,710032 |
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| 基金项目： | 国家自然科学基金资助项目:(编号30271292) |
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| 摘 要： | 目的:用不同方法去除猪胸主动脉壁细胞,并比较去细胞效果,为组织工程瓣膜或带瓣管道提供良好的实验材料。方法:采用十二烷基硫酸钠、胆脂酸钠、曲拉通（TritonX100）制备猪胸主动脉脱细胞基质。将实验分为十二烷基硫酸钠组、胆脂酸钠、曲拉通组和空白对照组。标本进行大体、光镜、电镜观察,力学性能的检测并做比较。结果:胆脂酸钠无法完全脱除主动脉壁内细胞,十二烷基硫酸钠和曲拉通都能完全脱除猪胸主动脉细胞,曲拉通较好地保持了胶原纤维和弹性纤维的原有排列和分布,并有较好的力学性能。后两者脱细胞时间无显著差别。结论:曲拉通可以成功用于制备大血管脱细胞基质,为组织工程瓣膜或带瓣管道的研究提供材料。
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| 关 键 词： | 胰蛋白酶 曲拉通X100 组织工程 血管 脱细胞基质 |
| 文章编号： | 1009-7236(2005)03-0207-03 |
| 修稿时间： | 2004-07-06 |
Primary study on cell extraction from porcine thoracic aortas |
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| GU Chun-hu,Liu Wei-yong,ZHANG Jin-bao,WANG Yun-ya,LIU Yang,TAN Hong-Mei,Su Xue-jia. Primary study on cell extraction from porcine thoracic aortas[J]. Chinese Heart Journal, 2005, 17(3): 207-209. DOI: 10.13191/j.chj.2005.03.10.guchh.004 |
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| Authors: | GU Chun-hu Liu Wei-yong ZHANG Jin-bao WANG Yun-ya LIU Yang TAN Hong-Mei Su Xue-jia |
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| Abstract: | AIM: To investigate methods to remove cellular compenents from porcine thoracic aortas,resulting scaffolds of acellular tissue matrix(ACTM) for tissue engneering of heart valve or vessel. METHODS:Different detergents (Triton X-100,Sodium dodecyl sulfate and sodium deoxycholate)and enzyme (trypsin) extraction was practiced in this study. According to the different detergents, specimens of porcine thoracic aortas devided into three groups(Sodium dodecyl sulfate,sodium deoxycholate,Triton X-100). Specimens were observed grossly and the changes of mechanical properties were also studied.Haematoxylin-eosin a was performed to confirm the removal of cells. Transmission electron microscope was used to observe the integrity of collagen and elastin. RESULTS:The cells were removed effectively from porcine thoracic aortas by Triton X-100 and sodium dodecyl sulfate, and histological analysis showed that the major structural compenents of porcine thoracic aortas decelluarized by Triton X-100 had been maintained.While sodium deoxycholate were failed for porcine thoracic aortas decellularization. the collagen and elastin were kept intact. CONCLUSION:ACTM of vessel can be obtained successfully through this procedure by Triton X-100. |
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| Keywords: | trypsin triton X-100 tissue engineering vessel acellular tissue matrix |
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