| PS-PVP静电纺丝膜抑制牙龈卟啉单胞菌黏附的作用研究 |
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| 引用本文： | 国嘉,陈佳敏,李雨庆,周学东. PS-PVP静电纺丝膜抑制牙龈卟啉单胞菌黏附的作用研究[J]. 四川大学学报(医学版), 2021, 52(5): 754-758. DOI: 10.12182/20210960102 |
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| 作者姓名： | 国嘉 陈佳敏 李雨庆 周学东 |
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| 作者单位： | 口腔疾病研究国家重点实验室 国家口腔疾病临床医学研究中心 四川大学华西口腔医院 成都 610041 |
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| 基金项目： | 国家自然科学基金（No. 31870065）资助 |
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| 摘 要： | 目的 探究聚苯乙烯(PS)及PS-聚乙烯吡咯烷酮(PVP)静电纺丝材料对牙龈卟啉单胞菌黏附能力的影响.方法 采用不锈钢针头及高压电场制备PS及PS-PVP静电纺丝材料;通过扫描电子显微镜观察不同材料表面的牙龈卟啉单胞菌生长黏附状态;通过菌落形成单位(colony forming unit,CFU)测定不同材料表面的牙龈...
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| 关 键 词： | 牙龈卟啉单胞菌 黏附 静电纺丝 表面电位 |
| 收稿时间： | 2021-07-14 |
Study on the Effect of Polystyrene-Polyvinylpyrrolidone Electrospun Fibre in Inhibiting the Adhesion of Porphyromonas gingivalis |
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| GUO Jia,CHEN Jia-min,LI Yu-qing,ZHOU Xue-dong. Study on the Effect of Polystyrene-Polyvinylpyrrolidone Electrospun Fibre in Inhibiting the Adhesion of Porphyromonas gingivalis[J]. Journal of Sichuan University. Medical science edition, 2021, 52(5): 754-758. DOI: 10.12182/20210960102 |
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| Authors: | GUO Jia CHEN Jia-min LI Yu-qing ZHOU Xue-dong |
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| Affiliation: | State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China |
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| Abstract: | Objective To explore the effect of polystyrene (PS) and PS-polyvinylpyrrolidone (PVP) electrospun materials on the adhesion ability of Porphyromonas gingivalis (P. gingivalis), a common periodontal pathogen. Methods PS and PS-PVP electrospun materials were prepared with stainless steel needles in high-voltage electric field. The growth and adhesion of P. gingivalis on the surface of different materials were observed with scanning electron microscope (SEM). The changes in the amount of P. gingivalis biofilm formed on the surface of different materials were measured according to viable colony forming units (CFU). The effect of surface charge of the different materials on the adhesion ability of P. gingivalis was determined through changing the charge properties on the surface of the electrospun materials. Results SEM images showed that both PS and PS-PVP can be used to form electrospun fibers with a diameter of 0.2 μm. SEM images and CFU counts of the biofilm at 24 h and 48 h showed that there was a smaller amount of P. gingivalis biofilm on the surface of the two materials (P<0.05). After treatment with tetrabutylammonium bromide (TBAB), the surface charge of the PS-PVP electrospun material changed from being negatively charged to being positively charged, and the amount of bacterial adhesion on the surface increased significantly in comparison to that of untreated PS and PS-PVP materials (P<0.05). Conclusion PS and PS-PVP electrospun materials can be used to reduce the adhesion ability of P. gingivalis on the surface of different materials, and this ability may be related to the surface charge properties of the materials. |
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