| 共载吡柔比星和长春瑞宾混合胶束的构建及其对乳腺癌的治疗研究 |
| |
| 引用本文： | 陈丹,周红利,赵婷,郭晨琦,张荣苹,李佳,龚涛. 共载吡柔比星和长春瑞宾混合胶束的构建及其对乳腺癌的治疗研究[J]. 四川大学学报(医学版), 2021, 52(4): 612-618. DOI: 10.12182/20210760105 |
| |
| 作者姓名： | 陈丹 周红利 赵婷 郭晨琦 张荣苹 李佳 龚涛 |
| |
| 作者单位： | 四川大学华西药学院 靶向药物与释药系统教育部重点实验室 成都 610041;四川大学华西口腔医院 成都 610041 |
| |
| 基金项目： | 国家自然科学基金（No. 81872804）资助 |
| |
| 摘 要： | 目的 研究使用硫酸软骨素-胆固醇聚合物(CS-Chol)和二硬脂酰基磷脂酰乙醇胺-聚乙二醇(DSPE-mPEG2000)构建一种共载吡柔比星(pirarubicin,THP)和长春瑞宾(vinorelbine,VRL)的混合胶束(T+V-CS胶束),并对其治疗乳腺癌的效果进行评价.方法 用超声-透析法制备T+V-CS胶...
|
| 关 键 词： | 吡柔比星 长春瑞宾 胶束 乳腺癌 药效学 |
| 收稿时间： | 2021-02-01 |
Co-delivery of Pirarubicin and Vinorelbine by Micelles for the Treatment of Breast Cancer |
| |
| CHEN Dan,ZHOU Hong-li,ZHAO Ting,GUO Chen-qi,ZHANG Rong-ping,LI Jia,GONG Tao. Co-delivery of Pirarubicin and Vinorelbine by Micelles for the Treatment of Breast Cancer[J]. Journal of Sichuan University. Medical science edition, 2021, 52(4): 612-618. DOI: 10.12182/20210760105 |
| |
| Authors: | CHEN Dan ZHOU Hong-li ZHAO Ting GUO Chen-qi ZHANG Rong-ping LI Jia GONG Tao |
| |
| Affiliation: | 1.Key Laboratory of Drug Targeting and Delivery System of the Ministry of Education, West China School of Pharmacy, Sichuan University, Chengdu 610041, China |
| |
| Abstract: | Objective To develop a pirarubicin (THP) and vinorelbine (VRL) codelivery nano-micellar system (T+V-CS micelles) of pirarubicin (THP) and vinorelbine (VRL) by using chondroitin sulfate-cholesterol polymers (CS-Chol) and DSPE-mPEG2000 and to evaluate the therapeutic efficacy of the codelivery nano-micelles in breast cancer treatment. Methods T+V-CS micelles were prepared by ultrasonic-dialysis method, and the physicochemical characterization were evaluated using multiple technological means. The anti-tumor efficacy of T+V-CS micelles in vitro was evaluated by MTT assay and cell cycle arrest analysis. Evaluation of the therapeutic effect of T+V-CS micelles in vivo was carried out on xenograft 4T1 murine breast cancer bearing BALB/c mice model. Results T+V-CS micelles displayed a nearly spherical shape when observed through transmission electron microscope. The particle size and polydispersity indexes (PDI) of T+V-CS micelles was (155.5±4.5) nm and 0.170±0.003 respectively, while the Zeta potential was (?23.0±0.9) mV. Meanwhile, T+V-CS micelles demonstrated high encapsulation efficiency of (81.87±2.56)% for THP and (87.54±2.82)% for VRL and a high overall drug loading efficiency of (10.20±1.20)%. In vitro and in vivo studies of the therapeutic efficacy of breast cancer showed that T+V-CS micelles had synergistic anti-tumor effect and induced increased G2/M cell cycle arrest in 4T1 cells, which could significantly inhibit tumor growth and prolong survival compared with the therapeutic efficacy of micelles loaded with a single kind of drug or free drug solutions. Conclusion The study showed that T+V-CS micelles had excellent anti-tumor effect, offering a reference to the clinical treatment of breast cancer. |
| |
| Keywords: | |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《四川大学学报(医学版)》浏览原始摘要信息 |
|
点击此处可从《四川大学学报(医学版)》下载免费的PDF全文 |
|
