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| 气相色谱法测定糖平煎复方中苍术酮含量 | |
| 引用本文： | 田嘉铭,于小红,王银月,王旭,王治宝,王金,卢金铭. 气相色谱法测定糖平煎复方中苍术酮含量[J]. 神经药理学报, 2020, 10(5): 14. DOI: 10.3969/j.issn.2095-1396.2020.05.004 |
| 作者姓名： | 田嘉铭 于小红 王银月 王旭 王治宝 王金 卢金铭 |
| 作者单位： | 1. 河北北方学院药学系，张家口，075000，中国2. 河北省神经药理学重点实验室，张家口，075000，中国3. 河北建筑工程学校，保定，071000，中国 |
| 基金项目： | 张家口市科技局项目（No.1411063C） |
| 摘 要： | 目的：建立气相色谱法测定糖平煎中苍术酮含量的方法。方法：色谱柱为PEG-20M 毛细管色谱柱（30 m×0.25 mm×0.25 μm），采用程序升温，进样口温度为290 ℃，检测器温度为300 ℃，检测器为氢火焰离子化检测器（flame ionization detector，FID），载气为氮气，流速1 mL·min-1，内标物质为萘。结果：被测物苍术酮与内标物质萘分离度均良好，平均回收率为100.1%，RSD 为2.64%，糖平煎中苍术酮含量为1.13 mg·g-1。结论：该研究建立的气相色谱方法简便、灵敏度高、数据准确，可用于糖平煎复方中苍术酮含量的测定。 |
| 关 键 词： | 糖平煎 苍术酮 含量测定 气相色谱法 |
Determination of Atractone in Tangpingjian by GasChromatography |
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| TIAN Jia-ming,YU Xiao-hong,WANG Yin-yue,WANG Xu,WANG Zhi-bao,WANG Jin,LU Jin-ming. Determination of Atractone in Tangpingjian by GasChromatography[J]. Journal of Hebei North University(Medical Edition), 2020, 10(5): 14. DOI: 10.3969/j.issn.2095-1396.2020.05.004 | |
| Authors: | TIAN Jia-ming YU Xiao-hong WANG Yin-yue WANG Xu WANG Zhi-bao WANG Jin LU Jin-ming |
| Affiliation: | 1. Department of Pharmacy，Hebei North University，Zhangjiakou，075000，China 2. Hebei Provincial Key Laboratory of Neuropharmacology，Zhangjiakou，075000，China 3. Hebei Building Engineering School，Baoding，071000，China |
| Abstract: | Objective：To establish a gas chromatography method for the determination of atractylone in TangpingJian. Method：PEG-20M capillary column （30 m×0.25 mm×0.25 μm） was used as the chromatographic column. The temperature was programmed with the inlet temperature of 290 ℃ and detector temperature of 300 ℃，and the detector was hydrogen flame ionization detector （FID）. The carrier gas was nitrogen with the flow rate of 1 mL·min-1， and naphthalene was used as the internal standard substance. Results：The separation of the atractylone and the internal standard substance was good，and the average recovery rate and RSD was 100.1% and 2.64%，respectively. The atractylone content in the Tangpingjian was 1.13 mg·g-1. Conclusion：The established gas chromatographic method is simple，highly sensitive and accurate. It can be used for the detection of atractylone content in TangpingJian. |
| Keywords: | tangpingjian atractylone determination gas chromatography |
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