| 携带细胞周期调控基因P14ARF的重组腺病毒的构建 |
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| 引用本文： | 鲜均明,周光耀,梁传余,刘世喜. 携带细胞周期调控基因P14ARF的重组腺病毒的构建[J]. 华西口腔医学杂志, 2006, 24(3): 261-264 |
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| 作者姓名： | 鲜均明 周光耀 梁传余 刘世喜 |
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| 作者单位： | 四川大学基础与法医学院,人体解剖学教研室,四川,成都,610041;四川大学华西医院,耳鼻咽喉科,四川,成都,610041 |
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| 基金项目： | 国家自然科学基金;四川省青年科技基金 |
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| 摘 要： | 目的 构建携带参与细胞周期调控的p14ARF基因的重组腺病毒载体。方法 将野生型p14ARF全长cDNA基因片段插入腺病毒载体质粒pAdTrack-CMV，与骨架质粒在大肠杆菌BJ5183胞内进行同源重组，经293细胞包装、扩增后得到携带p14ARF的重组腺病毒AdEasy-GFP-p14ARF。结果 成功构建了AdEasy-GFP-p14ARF的重组腺病毒载体系统，经测定腺病毒载体滴度达2.3×109 efu/mL。结论 构建的重组腺病毒AdEasy-GFP-p14ARF可望有效地将p14ARF基因导入喉癌细胞株/组织内，为进一步研究喉癌细胞信号转导干预治疗机制提供实验基础。
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| 关 键 词： | 细胞信号转导 重组腺病毒 基因治疗 喉癌 |
| 文章编号： | 1000-1182（2006）03-0261-04 |
| 收稿时间： | 2005-09-21 |
| 修稿时间： | 2005-12-23 |
Construction of Recombinant Adenovirus Vector Carrying Cell Cycle Controlling Gene-p14ARF |
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| XIAN Jun-ming,ZHOU Guang-yao,LIANG Chuan-yu,LIU Shi-xi. Construction of Recombinant Adenovirus Vector Carrying Cell Cycle Controlling Gene-p14ARF[J]. West China journal of stomatology, 2006, 24(3): 261-264 |
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| Authors: | XIAN Jun-ming ZHOU Guang-yao LIANG Chuan-yu LIU Shi-xi |
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| Affiliation: | 1. Dept. of Human Anatomy, Institute of Preclinical Medicine and Forensic Medicine, Sichuan University, Chengdu 610041, China; 2. Dept. of Otolaryngology, West China Hospital, Sichuan University, Chengdu 610041, China |
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| Abstract: | OBJECTIVE: The recombinant adenovirus vector carrying p14ARF gene was constructed for using in the interference therapy in signal transduction of laryngeal squamous cell carcinoma. METHODS: The total cDNA fragment of p14ARF was cloned into the shuttle plasmid pAdTrack-CMV, with the resultant plasmid and the backbone plasmid pAdEasy-1, the homologous recombination took place in the E.Coli BJ5183 and the recombinant adenoviral plasmid was generated. The adenoviruses were packaged and amplified in the 293 cells. Then the viral titer was checked by GFP. RESULTS: The recombinant adenovirus vector carrying p14ARF was constructed successfully. The viral titer was 2.3 x 10(9). CONCLUSION: The recombinant adenovirus vector could introduce p14ARF gene into the laryngeal squamous cell carcinoma line or tumor tissue effectively, which would provide experimental basis for the mechanisms and further study of the interference therapy in signal transduction of laryngeal squamous cell carcinoma. |
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| Keywords: | cell signal transduction recombinant adenovirus gene therapy laryngeal squamous cell carcinoma |
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