| 国产^13C-美沙西丁呼气试验对小鼠急性肝损伤的评价 |
| |
| 引用本文： | 陈素芸,姜磊,管樑,卢伟京,卢浩,杨维成,李彪,李培勇,杜晓宁,李良君,朱承谟. 国产^13C-美沙西丁呼气试验对小鼠急性肝损伤的评价[J]. 上海交通大学学报(医学版), 2010, 30(1): 55-58 |
| |
| 作者姓名： | 陈素芸 姜磊 管樑 卢伟京 卢浩 杨维成 李彪 李培勇 杜晓宁 李良君 朱承谟 |
| |
| 作者单位： | 1. 上海交通大学医学院瑞金医院核医学科,上海,200025
2. 上海化工研究院,上海,200062 |
| |
| 基金项目： | 国家高技术研究发展计划(八六三计划)，上海市科委资助项目 |
| |
| 摘 要： | 目的建立一套简单易操作并在小鼠活体上进行的^13C呼气试验检测系统，并探讨国产^13C-美沙西丁呼气试验（^13C-MBT）对小鼠急性肝损伤的诊断价值。方法^13C-美沙西丁经扑热息痛甲烷化合成标记。采用四氯化碳（CCl4）腹腔注射制作小鼠急性肝损伤模型，造模后饲养1个月复制小鼠急性肝损伤恢复模型，行肝脏病理组织学检查和肝功能血液生化学指标检测，于多个时间点采集活体小鼠^13C-美沙西丁灌胃后呼出的气体，以红外线能谱仪（IRIS）检测并绘制呼气峰值（DOB）曲线。结果正常对照组小鼠于灌胃给药后6～8min时呼气^13C达到峰值（51．9±2．04），随后缓慢下降至本底值；模型组于灌胃给药后16min左右达到峰值（26．37±5．74），随后亦缓慢下降至本底值，两组间差异有统计学意义（P〈0．05）。当急性肝损伤恢复模型组和对照组小鼠在相同条件下饲养1个月后，重复^13C-美沙西丁呼气检测后两组DOB峰值及达峰时间差异无统计学意义（P〉0．05）。结论^13C—MBT可在小鼠活体状态下准确、方便地采集和检测呼出气体的^13CO2变化，并正确评估其肝功能的损伤和恢复情况。
|
| 关 键 词： | 呼气试验 小鼠 国产^13C-美沙西丁 |
Evaluation of mouse acute liver damage by breath test with domestically synthesized ~(13)C-methacetin |
| |
| CHEN Su-yun,JIANG Lei,GUAN Liang,LU Wei-jing,LU Hao,YANG Wei-cheng,LI Biao,LI Pei-yong,DU Xiao-ning,LI Liang-jun,ZHU Cheng-mo. Evaluation of mouse acute liver damage by breath test with domestically synthesized ~(13)C-methacetin[J]. Journal of Shanghai Jiaotong University:Medical Science, 2010, 30(1): 55-58 |
| |
| Authors: | CHEN Su-yun JIANG Lei GUAN Liang LU Wei-jing LU Hao YANG Wei-cheng LI Biao LI Pei-yong DU Xiao-ning LI Liang-jun ZHU Cheng-mo |
| |
| Affiliation: | 1. Department of Nuclear Medicine, Ruifin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200025, China; 2. Shanghai Research Institute of Chemical Industry, Shanghai 200062, China) |
| |
| Abstract: | Objective To establish a convenient ~(13)C-breath test system in live mice,and investigate the value of ~(13)C-methacetin breath test(~(13)C-MBT) in the diagnosis of acute liver damage of mice with domestically synthesized ~(13)C-methacetin. Methods Domestically synthesized ~(13)C.methacetin was prepared from aeamol by methylation. Abdominal injection of CCl_4 was adopted to duplicate acute liver damage of mice,then the mice were housed under normal laboratory condition for a whole month to gain recovery,which were indentified by hepatic pathological examinations and biochemical teats of liver function.After fasting, the mice were orally administered ~(13)C-methacetin,and the expired air was collected at various time points. Infrared spectrometer was employed, and delm over baseline(DOB) curves of ~(13)C-exhalation were drawn. Results Six to eight min after administration of ~(13)C-methacetin,the rate of ~(13)C-exhalation peaked in control group(51.9±2.04), and decreased thereafter. Sixteen min after administration of ~(13)C-methacetin,the rate of ~(13)JC-exhalation peaked in model group(26.37±5.74), and decreased thereafter.There were significant differences between these two groups(P<0.05).There was no significant difference in peak value and time to reach the peak on DOB curves of ~(13)C-methacetin breath test after the two groups of mice were housed under the same condition for a month(P>0.05).Conclution ~(13)C-MBT facilitates the collection and evaluation of ~(13)CO_2 in the expired air of live mice,and yields precise reflection of alterations of liver function in acute liver injury and functional recovery. |
| |
| Keywords: | breath test mice domestically synthesized ~(13)C-methacetin |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
