| STAT3信号通路对大肠癌细胞VEGF表达的影响 |
| |
| 引用本文： | 张永东,高源,黄海,乔志明,钱伟峰. STAT3信号通路对大肠癌细胞VEGF表达的影响[J]. 岭南现代临床外科, 2011, 11(6): 423-425. DOI: 10.3969/j.issn.1009-976X.2011.06.006 |
| |
| 作者姓名： | 张永东 高源 黄海 乔志明 钱伟峰 |
| |
| 作者单位： | 南京医科大学附属苏州医院 |
| |
| 摘 要： | 目的观察STAT3信号通路对大肠癌HT-29细胞生长及VEGF表达的影响。方法 pRNAT-shSTAT3重组质粒瞬时转染大肠癌HT-29细胞,实时定量PCR、Westernblot检测STAT3的表达情况,MTT检测细胞的生长情况,实时定量PCR、ELISA检测VEGF的表达。结果 pRNAT-shSTAT3重组质粒转染大肠癌细胞后,STAT3表达明显降低,细胞生长能力明显减弱,VEGF表达明显降低,与对照组相比差异显著(P<0.01)。结论干扰STAT3基因的表达可以有效抑制大肠癌HT-29细胞的生长及VEGF的表达。
|
| 关 键 词： | 大肠癌 STAT3信号通路 RNA干扰 shRNA |
| 收稿时间： | 2011-10-21 |
Effects of STAT3 signaling pathway on VEGF expression in human colorectal cancer cells |
| |
| Zhang Yongdong,Gao Yuan,Huang Hai,Qiao zhiming,Qian Weifeng. Effects of STAT3 signaling pathway on VEGF expression in human colorectal cancer cells[J]. Lingnan Modern Clinics in Surgery, 2011, 11(6): 423-425. DOI: 10.3969/j.issn.1009-976X.2011.06.006 |
| |
| Authors: | Zhang Yongdong Gao Yuan Huang Hai Qiao zhiming Qian Weifeng |
| |
| Affiliation: | Zhang Yongdong,Gao Yuan,Huang Hai,Qiao zhiming,Qian Weifeng,Department of General Surgery,Suzhou Municipal Hospital,Suzhou,Jiangsu 215002 |
| |
| Abstract: | Objective To investigate the effects of STAT3 signaling pathway on cells proliferation and VEGF expressing in human colorectal cancer HT-29 cells.Methods HT-29 cells were transfected with the pRNAT-shSTAT3 recombinant plasmids.Real time quantitative PCR and Westernblot were used to quantify the expression of STAT3 gene.The cell growth was assessed by MTT assay.VEGF expression was detected by Real time quantitative PCR and ELISA.Results STAT3 shRNA inhibited the expression of STAT3 gene in HT-29 cells.Transf... |
| |
| Keywords: | Colorectal cancer STAT3 signaling pathway RNA interfering Short hairin RNA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《岭南现代临床外科》浏览原始摘要信息 |
|
点击此处可从《岭南现代临床外科》下载全文 |
|
