The Saccharomyces cerevisiae Fin1 protein forms cell cycle-specific filaments between spindle pole bodies |
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| Authors: | van Hemert Martijn J Lamers Gerda E M Klein Dionne C G Oosterkamp Tjerk H Steensma H Yde van Heusden G Paul H |
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| Affiliation: | Section Yeast Genetics, Institute of Molecular Plant Sciences, Leiden University, Wassenaarseweg 64, 2333AL, Leiden, The Netherlands. |
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| Abstract: | The FIN1 gene from the yeast Saccharomyces cerevisiae encodes a basic protein with putative coiled-coil regions. Here we show that in large-budded cells a green fluorescent protein-Fin1 fusion protein is visible as a filament between the two spindle pole bodies. In resting cells the protein is undetectable, and in small-budded cells it is localized in the nucleus. During late mitosis it localizes on the spindle pole bodies. Filaments of cyano fluorescent protein-tagged Fin1 colocalize with filaments of green fluorescent protein-tagged Tub1 only in large-budded cells. By electron and atomic force microscopy we showed that purified recombinant Fin1p self-assembles into filaments with a diameter of approximately 10 nm. Our results indicate that the Fin1 protein forms a cell cycle-specific filament, additional to the microtubules, between the spindle pole bodies of dividing yeast cells. |
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