| 青光眼术后干眼患者泪液中MAPK、Sirt1水平与泪膜功能的相关性分析 |
| |
| 引用本文： | 郭宣妮,姜春辉,朱安泰,王燕,李春艳,王娟妮. 青光眼术后干眼患者泪液中MAPK、Sirt1水平与泪膜功能的相关性分析[J]. 眼科新进展, 2020, 0(7): 663-666. DOI: 10.13389/j.cnki.rao.2020.0152 |
| |
| 作者姓名： | 郭宣妮 姜春辉 朱安泰 王燕 李春艳 王娟妮 |
| |
| 作者单位： | 712000 陕西省咸阳市，咸阳市中心医院眼科 |
| |
| 摘 要： |
|
| 关 键 词： | 青光眼 小梁切除术 干眼 丝裂原活化蛋白激酶 沉默信息调节因子相关酶1 泪膜功能 |
Correlations between MAPK,Sirt1 levels in lacrimal gland and tear film function in dry eye patients after glaucoma surgery |
| |
| GUO Xuanni,JIANG Chunhui,ZHU Antai,WANG Yan,LI Chunyan,WANG Juanni. Correlations between MAPK,Sirt1 levels in lacrimal gland and tear film function in dry eye patients after glaucoma surgery[J]. Recent Advances in Ophthalmology, 2020, 0(7): 663-666. DOI: 10.13389/j.cnki.rao.2020.0152 |
| |
| Authors: | GUO Xuanni JIANG Chunhui ZHU Antai WANG Yan LI Chunyan WANG Juanni |
| |
| Affiliation: | Department of Ophthalmology，Xianyang Central Hospital, Xianyang 712000, Shaanxi Province, |
| |
| Abstract: | Objective To analyze the relationship between mitogen-activated protein kinase (MAPK), silment information regulator factor related enzymes 1 (Sirt1) in lacrimal gland and tear film function in dry eye patients after glaucoma surgery. Methods A total of 50 dry eye patients after glaucoma surgery who were confirmed in our hospital from September 2015 to December 2017 were enrolled as dry eye group. Another 50 patients who were not complicated with ocular disease after glaucoma trabeculectomy in the same period were enrolled as control group. Western blot was used to detect the protein expression levels of p38MAPK, p-p38MAPK, JNK, p-JNK, ERK1/2, p-ERK1/2 and Sirt1 in the tears of the two groups of patients. RT-PCR was used to detect the expression levels of p38MAPK, JNK, ERK1/2 and Sirt1mRNA in the tears of the two groups of patients. The break-up time (BUT), Schirmer I test (SIt) and fluorescein staining (FL) score in both groups were detected. Pearson correlation analysis was performed to analyze correlation between above proteins and tear film function. Results Compared with control group, expression levels of p-p38MAPK, p-JNK and p-ERK1/2 in tears of dry eye group after glaucoma surgery were significantly up-regulated (all P<0.05), while expression levels of Sirt1 mRNA and protein were significantly down-regulated (all P<0.05), and there was no significant difference in the expression levels of p38MAPK, JNK and ERK1/2 mRNA (all P>0.05); BUT and SIt were significantly decreased (both P<0.001), and FL score was significantly increased (P<0.001). The expression level of p-p38MAPK, p-JNK and p-ERK1/2 in tears of dry eye group was significantly negatively correlated with BUT and SIt (all P<0.001), while significantly positively correlated with FL score (all P<0.001). The expression level of Sirt1 in tears was significantly positively correlated with BUT and SIt (both P<0.001), while significantly negatively correlated with FL score (P<0.001). Conclusion The levels of MAPK and Sirt1 in tears of dry eye patients after glaucoma surgery are significantly correlated with tear film function. |
| |
| Keywords: | glaucoma trabeculectomy dry eye mitogen-activated protein kinase silment information regulator factor related enzymes 1 tear film function |
|
| 点击此处可从《眼科新进展》浏览原始摘要信息 |
|
点击此处可从《眼科新进展》下载免费的PDF全文 |
|
