| 血管内皮舒张因子对豚鼠耳蜗功能的影响 |
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| 引用本文： | 贾学斌,李兴启,张瀛. 血管内皮舒张因子对豚鼠耳蜗功能的影响[J]. 中国耳鼻咽喉头颈外科, 2011, 18(10): 535-539 |
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| 作者姓名： | 贾学斌 李兴启 张瀛 |
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| 作者单位： | 1. 山西省大同市第三医院耳鼻喉科;2. 中国人民解放军总医院耳研所;3. 昆明医学院第一附属医院耳鼻喉科; |
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| 基金项目： | 国家自然科学基金资助项目（30070812） |
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| 摘 要： | 目的 探讨血管内皮性一氧化氮合酶(endothelial nitric oxide synthase,eNOS)对耳蜗电位的影响.方法 健康豚鼠100只,随机等分为10组:①人工外淋巴液组;②L-精氨酸组;③Ca2+-ATP酶抑制剂组;④Ca2+-ATP酶抑制剂+L-精氨酸组;⑤Ca2+-ATP酶抑制剂+环磷酸鸟苷(c...
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| 关 键 词： | 一氧化氮 一氧化氮合酶 耳蜗微音电位 豚鼠 |
| 收稿时间： | 2010-10-15 |
Effects of blood circulation on cochlear microcirculation in the cochlea of guinea pigs |
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| JIA Xuebin,LI Xingqi,ZHANG Ying. Effects of blood circulation on cochlear microcirculation in the cochlea of guinea pigs[J]. Chinese Archives of Otolaryngology-Head and Neck Surgery, 2011, 18(10): 535-539 |
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| Authors: | JIA Xuebin LI Xingqi ZHANG Ying |
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| Affiliation: | 1 Department of Otolaryngology,Datong Third Hospital,Datong,Shanxi,037008,China;2 Institute of Otolaryngology,General Hospital of PLA,Beijing,100039,China;3 Department of Otolaryngology,the First Affiliated Hospital,Kunming Medical University,Kunming,Yunnan,650011,China) |
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| Abstract: | OBJECTIVE To study the cochlear microcirculation effects of endothelial nitric oxide synthase（eNOS）of guinea pigs.METHODS Total 100 guinea pigs were divided into 10 groups as follows： ①artificial perilymph;②Ca2＋-ATPase inhibitor;③ L-arginine;④Ca2＋-ATPase inhibitor＋L-arginine;⑤Ca2＋ATPase inhibitor＋cGMP;⑥Ca2＋-ATPase inhibitor＋Larginine＋Non-selective NOS inhibitor;⑦nNOS inhibitor;⑧nNOS inhibitor＋Ca2＋-ATPase inhibitor;⑨ nNOS inhibitor＋Ca2＋-ATPase inhibitor＋L-arginine;⑩ nNOS inhibitor＋Ca2＋-ATPase inhibitor＋L-arginine＋eNOS inhibitor.Perilymphs of guinea pig were perfused with the drugs mentioned above at a rate of 2.5 μl/min for 2 hours.The compound action potential（CAP）was evoked by click,and the cochlea microphnics（CM）evoked by tone burst（1 kHz,rise and fall 2 ms,and plateau 20 ms）.The CAP and CM were recorded from the round window（RM） every 30 minutes.RESULTS The average threshold shift of CAP after perfusing Ca2＋-ATPase inhibitor was 28.5 dB SPL,and its improvement by L-arginine in group 4 was 9 dB SPL,similar to group 5.The threshold shift of CAP in group 8 was 43 dB SPL,and additional improvement of 7 dB SPL showed out after L-arginine added.Compared with group 9,more 6 dB SPL of improvement was got in group 10 after eNOS inhibitor added which has no obvious differences with group 8.Immunohistochemical results showed that L-arginine can improve the eNOS expression on cochlear striae vascularis.CONCLUSION ENOS can regulate cochlear microcirculation by adjusting contract and dilation of small artery on cochlear striae vascularis. |
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| Keywords: | Nitric Oxide Nitric-Oxide Synthase Cochlear Microphonic Potential Guinea Pigs |
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