| 益肾活血提取液对同型半胱氨酸所致的兔VSMCs增殖的抑制作用 |
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| 引用本文： | 来丽萍,张继东,李丽珍,刘粉叶. 益肾活血提取液对同型半胱氨酸所致的兔VSMCs增殖的抑制作用[J]. 山东大学学报(医学版), 2007, 45(10): 1011-1015 |
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| 作者姓名： | 来丽萍 张继东 李丽珍 刘粉叶 |
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| 作者单位： | 山东大学齐鲁医院,中医科,济南,250012;山东大学齐鲁医院,肿瘤中心免疫室,济南,250012 |
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| 摘 要： | 目的：探讨益肾活血提取液（YSHXC）对同型半胱氨酸（HCY）诱导的兔主动脉平滑肌细胞（VSMCs）增殖的影响。方法：组织贴块法体外原代培养兔VSMCs，建立HCY诱导的VSMCs增殖模型并分组：正常组、HCY刺激组、HCY+YSHXC组、HCY+叶酸组。采用MTT法检测细胞的增殖活度，免疫组化技术检测增殖细胞核抗原（PCNA）的表达，并采用流式细胞术检测细胞周期。结果：YSHXC及叶酸均能降低PCNA的表达（P<0.01），且中药组低于叶酸组（P<0.05）。细胞周期也有相应的变化，与HCY组相比，YSHXC组G0/G1期细胞比例显著升高，S期与G2/M期细胞显著降低（P<0.01），基本接近正常对照组（P>0.05）。结论：YSHXC能抑制HCY诱导的VSMCs增殖，下调细胞内PCNA的表达，这可能与YSHXC推动细胞周期时相的改变有关。
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| 关 键 词： | 益肾活血提取液 血管平滑肌细胞 增殖 细胞周期 增殖细胞核抗原 |
| 文章编号： | 1671-7554（2007）10-1011-05 |
| 收稿时间： | 2007-06-17 |
| 修稿时间： | 2007-06-17 |
Inhibitory effect of the Yishenhuoxue Capsule on the proliferati on of vascular smooth muscle cells induced by HCY in rabbits |
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| LAI Li-ping,ZHANG Ji-dong,LI Li-zhen,LIU Fen-ye. Inhibitory effect of the Yishenhuoxue Capsule on the proliferati on of vascular smooth muscle cells induced by HCY in rabbits[J]. Journal of Shandong University:Health Sciences, 2007, 45(10): 1011-1015 |
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| Authors: | LAI Li-ping ZHANG Ji-dong LI Li-zhen LIU Fen-ye |
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| Affiliation: | 1. Department of Traditional Chinese Medicine; 2. Department of Immunity, Qilu Hospital of Shandong University, Jinan 250012， Shandong， China |
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| Abstract: | Objective To explore the effects of the Yishenhuoxue Capsule(YSHXC) on the proliferation of vascular smooth muscle cells(VSMCs) induced by HCY in rabbits.Methods VSMCs were cultured by tissue explants in vitro and proliferation was induced by HCY.Cells were divided into four groups: the control group, the HCY group,the Yishenhuoxue group and the folic acid group.The proliferation of cells,the expression of PCNA and cell cycles were determined by MTT,immunohistochemistry and flow cytometric technique.Results After being separately incubated with Yishenhuoxue and folic acid,the expression of PCNA was effectively reduced(P<0.01) and that was especially in the Yishenhuoxue group(P<0.05).Cell cycles were also homologically changed.Compared with the HCY group,the cell number of the Yishenhuoxue group in the G0/G1 period was increased while in the S period was decreased,which showed that the cell number in the synthesis period of DNA went down remarkably(P<0.01).Conclusion Yishenhuoxue can significantly inhibit the proliferation of cells induced by HCY and bring down the level of PCNA,which may be related to a change of the cell cycle. Therefore,the Yishenhuoxue Capsule can delay the process of atheroscleorsis at an earlier period. |
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| Keywords: | Yishenhuoxue Capsule Vascular smooth muscle cells Proliferation Cell cycle Proliferating cell nuclear antigen |
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