| 间日疟原虫海南分离株乳酸脱氢酶基因的克隆与序列分析 |
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| 引用本文： | 吕刚,李静晶,赵世勇,芦亚君,范志刚. 间日疟原虫海南分离株乳酸脱氢酶基因的克隆与序列分析[J]. 中国寄生虫学与寄生虫病杂志, 2010, 28(1): 72-74 |
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| 作者姓名： | 吕刚 李静晶 赵世勇 芦亚君 范志刚 |
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| 作者单位： | 海南医学院寄生虫学教研室， 海口 571101 |
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| 基金项目： | 海南省自然科学基金(No30140); 国家自然科学基金(No30860070)~~ |
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| 摘 要： | 用PCR扩增间日疟原虫海南分离株基因组DNA中乳酸脱氢酶(LDH)全长基因,命名为PvLDH/HN(GenBank登录号为FJ527750)。生物信息学分析表明,PvLDH/HN全长951bp,编码316个氨基酸残基,与间日疟原虫SalvadorI株、Belem株LDH等分离株核苷酸序列同源性均为99.89%(950/951),氨基酸序列同源性均为100%(316/316)。拓扑结构分析显示,该蛋白具有2个α螺旋跨膜区域,可能是膜蛋白。三级结构模型显示主要抗原表位82～95aa位于蛋白表面,构成特异性底物结合环,提示该位点是可能的药物作用靶点及免疫诊断抗原表位。
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| 关 键 词： | 间日疟原虫；乳酸脱氢酶；克隆；序列分析 |
Molecular Cloning and Sequence Analysis of Lactate Dehydrogenase Gene from Plasmodium vivax Hainan Isolate |
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| LV Gang,LI Jing-Jing,ZHAO Shi-Yong,LU Ya-Jun,FAN Zhi-Gang. Molecular Cloning and Sequence Analysis of Lactate Dehydrogenase Gene from Plasmodium vivax Hainan Isolate[J]. Chinese Journal of Parasitology and Parasitic Diseases, 2010, 28(1): 72-74 |
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| Authors: | LV Gang LI Jing-Jing ZHAO Shi-Yong LU Ya-Jun FAN Zhi-Gang |
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| Affiliation: | (Department of Parasitology,Hainan Medical College,Haikou 571101,China) LV Gang,LI Jing-Jing,ZHAO Shi-Yong,LU Ya-Jun,FAN Zhi-Gang |
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| Abstract: | Full length sequence of lactate dehydrogenase gene was amplified by PCR from the genomic DNA of Plasmodium vivax Hainan isolate, and named as PvLDH/HN （GenBank No. FJ527750）. Sequence analysis showed that the gene had 951 bp, coding 316 aa. Compared with PvLDH/Salvador and PvLDH/Belem, the nucleotide sequence homology of PvLDH/HN was both 99.89%, while the homology of amino acid sequence was 100%. Topology analysis showed that the protein had two transmembrane α-helices, which suggested that the protein might be a membrane protein. The major antigen epitope regions （82-95aa） was presented on the protein surface, and formed the specific substrate binding loop, which revealed that it might be an ideal target site for drug action and immunodiagnosis. |
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| Keywords: | Plasmodium vivax Lactate dehydrogenase Clone Sequence analysis |
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