| 骨髓间充质干细胞移植联合益母草碱治疗宫腔粘连 |
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| 引用本文： | 王冰玉,姬 霞. 骨髓间充质干细胞移植联合益母草碱治疗宫腔粘连[J]. 中国组织工程研究, 2016, 20(32): 4771-4777. DOI: 10.3969/j.issn.2095-4344.2016.32.009 |
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| 作者姓名： | 王冰玉 姬 霞 |
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| 作者单位： | 河南省中医院妇产科，河南省郑州市 450002 |
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| 基金项目： | 河南省中医药管理局科研课题(2014ZY02013) |
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| 摘 要： |
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| 关 键 词： | 干细胞 移植 益母草碱 骨髓间充质干细胞 宫腔粘连 转化生长因子β Smad3 干扰素γ |
Leonurine combined with bone marrow mesenchymal stem cell transplantation in the treatment of intrauterine adhesions |
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| Wang Bing-yu,Ji Xia. Leonurine combined with bone marrow mesenchymal stem cell transplantation in the treatment of intrauterine adhesions[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(32): 4771-4777. DOI: 10.3969/j.issn.2095-4344.2016.32.009 |
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| Authors: | Wang Bing-yu Ji Xia |
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| Affiliation: | Department of Gynecology and Obstetrics, Henan Province Hospital of TCM, Zhengzhou 450002, Henan Province, China |
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| Abstract: | BACKGROUND:Increasing evidence has demonstrated that bone marrow mesenchymal stem cell (BMSC) transplantation can promote skin, liver and lung repair in animal models; and Leonurus sibiricus L. has the ability of promoting blood circulation and regulating menstruation.OBJECTIVE:To investigate the therapeutic effect of BMSC transplantation with leonurine on intrauterine adhesions (IUA) in rabbits and the relevant mechanism of action. METHODS:After modeled using dual injury method, rabbit models of IUA were randomly divided into five groups: sham-operated group (sham), model group (IUA), BMSC transplantation group, leonurine treatment group and combined treatment group (BMSCs+leonurine). Rabbits in the sham group were only given normal saline rinsing after hysterotomy, while those in the latter three groups were correspondingly given intrauterine BMSC transplantation or/and intragastric administration of 4 mg/kg leonurine for 14 days. Morphological changes of the endometrium were observed using hematoxylin-eosin staining, and expression levels of transforming growth factor β protein, Smad3 protein and interferon-γ mRNA were detected using immunohistochemical staining and real-time fluorescence quantitative PCR, respectively. RESULTS AND CONCLUSION:Compared with the model group, the degree of IUA was all significantly improved in the other groups, especially in the combined treatment group. Moreover, BMSC transplantation, leonurine treatment and their combined use all could inhibit IUA-induced increase of transforming growth factor β and Smad3 protein expression and IUA-induced decrease of interferon-γ mRNA level. Importantly, all these alternations were much more pronounced in the combined treatment group. Our results show that the combined use of BMSC transplantation and leonurine treatment can exert a synergistic effect in the improvement of IUA through the transforming growth factor β/Smad3 pathway. |
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| Keywords: | Mesenchymal Stem Cells Leonurus Uterus Transforming Growth Factor beta Smad3 Protein Tissue Engineering |
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