| 舒芬太尼复合丙泊酚全身麻醉在脐血间充质干细胞移植治疗脊髓损伤中的应用 |
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| 引用本文： | 李文亮,佟冬怡. 舒芬太尼复合丙泊酚全身麻醉在脐血间充质干细胞移植治疗脊髓损伤中的应用[J]. 中国组织工程研究, 2016, 20(45): 6739-6745. DOI: 10.3969/j.issn.2095-4344.2016.45.008 |
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| 作者姓名： | 李文亮 佟冬怡 |
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| 作者单位： | 1赤峰学院附属医院麻醉科，内蒙古自治区赤峰市 0240052中国医科大学附属盛京医院麻醉科，辽宁省沈阳市 110004 |
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| 摘 要： |
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| 关 键 词： | 干细胞 脐带脐血干细胞 丙泊酚 脐血间充质干细胞 舒芬太尼 脊髓损伤 干细胞移植 麻醉 |
Sufentanil combined with propofol anesthesia as an aid to umbilical cord blood mesenchymal stem cell transplantation for the treatment of spinal cord injury |
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| Li Wen-liang,Tong Dong-yi. Sufentanil combined with propofol anesthesia as an aid to umbilical cord blood mesenchymal stem cell transplantation for the treatment of spinal cord injury[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(45): 6739-6745. DOI: 10.3969/j.issn.2095-4344.2016.45.008 |
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| Authors: | Li Wen-liang Tong Dong-yi |
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| Affiliation: | 1Department of Anesthesiology, Affiliated Hospital of Chifeng University, Chifeng 024005, Inner Mongolia Autonomous Region, China
2Department of Anesthesiology, Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning Province, China |
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| Abstract: | BACKGROUND:Sufentanil and propofol are both found to have good neuroprotective effects on neurological damage in clinical practice.OBJECTIVE:To investigate the effect of propofol combined with sufentanil in umbilical cord blood mesenchymal stem cells transplantation for the treatment of spinal cord injury. METHODS:Sixty-five Wistar rats were selected to make animal models of acute spinal cord injury using Allen’s method. Six hours after modeling, these rats were randomly assigned into combined group (injection of 2×107/L human umbilical cord blood mesenchymal stem cell suspension (0.5 mL) plus injection of 1.0-1.5 mg/kg propofol and 0.5 μg/kg sufentanil via the tail vein), stem cell group (injection of 2×107/L human umbilical cord blood mesenchymal stem cell suspension (0.5 mL) via the tail vein), or control group (injection of 30 μL of LDMEM containing 5% fetal bovine serum). S100β protein level in serum was detected in each group at 15 and 60 minutes after injection. Motor function of rat in each group was assessed by Basso Beattie Bresnahan (BBB) scoring and incline plane test at 1, 2, 4, 6, 8 weeks after modeling. Pathological changes of the spinal cord were observed at 4 weeks after modeling. Expression of vascular endothelial growth factor was detected using western blot assay at 1 and 2 weeks after modeling. RESULTS AND CONCLUSION:After 15 and 60 minutes of intervention, S100β protein level was lowest in the combined group followed by the stem cell and control groups (P < 0.05). At 2, 4, 6, 8 weeks after modeling, scores on the incline plane test and BBB were ranked as follows: combined group > stem cell group > model group (P < 0.05). At 4 weeks after modeling, severe damage to the spinal cord and few nerve fibers were found in the control group; spinal cord hyperplasia and a few of regenerated axons and PKH-26-positive stem cells appeared in the stem cell group; while in the combined group, there were a large amount of PKH-26-positive stem cells and nerve axon-like structures. At 1 and 2 weeks after modeling, the highest protein level of vascular endothelial growth factor was found in the combined group followed by the stem cell group and control group (P < 0.05). To conclude, these findings indicate that propofol and sufentanil in umbilical cord blood mesenchymal stem cell transplantation therapy can promote the recovery of hindlimb function after spinal cord injury, thereby promoting the functional recovery of rats from spinal cord injury. |
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| Keywords: | Stem Cells Cord Blood Stem Cell Transplantation Propofol Sufentanil Tissue Engineering |
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