| 非接触共培养条件下骨髓间充质干细胞向类髓核细胞的诱导分化 |
| |
| 引用本文： | 武海军,银和平,胡继平,刘 聪,李树文,白 明,杜志才. 非接触共培养条件下骨髓间充质干细胞向类髓核细胞的诱导分化[J]. 中国组织工程研究, 2016, 20(45): 6706-6713. DOI: 10.3969/j.issn.2095-4344.2016.45.003 |
| |
| 作者姓名： | 武海军 银和平 胡继平 刘 聪 李树文 白 明 杜志才 |
| |
| 作者单位： | 1呼和浩特市第一医院，内蒙古自治区呼和浩特市 0100102内蒙古医科大学第二附属医院，内蒙古自治区呼和浩特市 010030 |
| |
| 基金项目： | 国家自然科学基金项目(gjzr12287) |
| |
| 摘 要： |
|
| 关 键 词： | 干细胞 骨髓干细胞 髓核细胞 骨髓间充质干细胞 非接触式共培养 兔 国家自然科学基金 |
Effect of indirect co-culture system on differentiation of bone marrow mesenchymal stem cells into nucleus pulposus-like cells |
| |
| Wu Hai-jun,Yin He-ping,Hu Ji-ping,Liu Cong,Li Shu-wen,Bai Ming,Du Zhi-cai. Effect of indirect co-culture system on differentiation of bone marrow mesenchymal stem cells into nucleus pulposus-like cells[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(45): 6706-6713. DOI: 10.3969/j.issn.2095-4344.2016.45.003 |
| |
| Authors: | Wu Hai-jun Yin He-ping Hu Ji-ping Liu Cong Li Shu-wen Bai Ming Du Zhi-cai |
| |
| Affiliation: | 1First Hospital of Hohhot, Hohhot 010010, Inner Mongolia Autonomous Region, China
2the Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China |
| |
| Abstract: | BACKGROUND:Mesenchymal stem cells (MSCs) for the treatment of degenerative disc diseases present a new therapeutic strategy for the structural and functional recovery of the degenerative intervertebral disc.OBJECTIVE:To study the differentiation potential of rabbit bone marrow MSCs (BMSCs) into nucleus pulposus-like cells. METHODS:Passage 3 BMSCs and nucleus pulosus cells (NPCs) extracted from rabbits were assigned into simple BMSCs culture, simple NPCs culture or co-culture group. In the former two groups, BMSCs or NPCs were cultured in 6-well culture plates. In the co-culture group, passage 3 BMSCs and NPCs were cultured on the upper or lower layer of the 6-well Transwell chamber, respectively. Cell morphology was observed by inverted contrast phase microscope. At 7 days of culture, immunocytochemistry, RT-PCR and western blot were used to examine the expression levels of type II collagen and aggrecan. RESULTS AND CONCLUSION:At 7 days of co-culture, BMSCs were polygonal or short spindle-shaped, with no fiber-like changes, and cell morphology was close to that of NPCs. Additionally, the expression levels of type II collagen and aggrecan in the BMSCs co-cultured with NPCs were similar to those in the NPCs cultured alone, but significantly higher than those in the BMSCs culture only. Overall, these results show that coculture of BMSCs with NPCs can induce BMSCs differentiating into an NPCs phenotype, indicating that BMSCs may provide sufficient sources of cells for the biological treatment of degenerative disc diseases. |
| |
| Keywords: | Bone Marrow Mesenchymal Stem Cells Coculture Techniques Intervertebral Disk Tissue Engineering |
|
| 点击此处可从《中国组织工程研究》浏览原始摘要信息 |
|
点击此处可从《中国组织工程研究》下载全文 |
|
