| 骨髓间充质干细胞移植治疗老年性痴呆模型大鼠的行为学检测 |
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| 引用本文： | 张 颖,郭建华,闫洪娟,罗秋华,李鑫萍,郭 玮,王海霞. 骨髓间充质干细胞移植治疗老年性痴呆模型大鼠的行为学检测[J]. 中国组织工程研究, 2016, 20(36): 5371-5377. DOI: 10.3969/j.issn.2095-4344.2016.36.008 |
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| 作者姓名： | 张 颖 郭建华 闫洪娟 罗秋华 李鑫萍 郭 玮 王海霞 |
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| 作者单位： | 邯郸市第一医院老年病二科，河北省邯郸市 056000 |
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| 基金项目： | 河北省医学科学研究重点课题(20130921) |
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| 摘 要： |
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| 关 键 词： | 干细胞 移植 骨髓间充质干细胞 老年性痴呆 学习记忆 大鼠 |
Bone marrow mesenchymal stem cell transplantation improves behavior performance of senile dementia rats |
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| Zhang Ying,Guo Jian-hua,Yan Hong-juan,Luo Qiu-hua,Li Xin-ping,Guo Wei,Wang Hai-xia. Bone marrow mesenchymal stem cell transplantation improves behavior performance of senile dementia rats[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(36): 5371-5377. DOI: 10.3969/j.issn.2095-4344.2016.36.008 |
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| Authors: | Zhang Ying Guo Jian-hua Yan Hong-juan Luo Qiu-hua Li Xin-ping Guo Wei Wang Hai-xia |
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| Affiliation: | Second Department of Geriatrics, First Hospital of Handan, Handan 056000, Hebei Province, China |
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| Abstract: | BACKGROUND:More recently, studies have demonstrated that bone marrow mesenchymal stem cells can be induced in vitro to differentiate into neuron-like cells that are used for in vivo transplantation to repair nerve damage.OBJECTIVE:To study the effect of bone marrow mesenchymal stem cell transplantation on learning and memory ability of senile dementia rats.METHODS:Thirty male Sprague-Dawley rats were randomly divided into three groups: normal control group, stem cell therapy group and model control group. Rats in the latter two groups were used to establish animal models of senile dementia by intracranial injection of β-amyloid 1-40. Three weeks after modeling, rats were given bilateral hippocampal injection of induced bone marrow mesenchymal stem cell suspension in the stem cell therapy group, whereas no treatment was given in the normal control and model control groups. Morris water maze test was used to detect learning and memory ability of rats, and rat’s brain tissues were detected pathologically using hematoxylin-eosin staining.RESULTS AND CONCLUSION:After modeling, the escape latency was higher and the cross-platform frequency was lower in the model control group compared with the normal control group. After cell transplantation, the escape latency and cross-platform frequency were gradually shortened and increased with time, respectively. Compared with the model control group, the learning and memory abilities of rats were improved in the stem cell therapy group. The brain tissues were relatively intact in structure and exhibited less cell degeneration and necrosis in the stem cell therapy group compared with the model control group. To conclude, bone marrow mesenchymal stem cell transplantation exerts certain therapeutic effects on senile dementia by effectively improving the learning and memory ability. |
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| Keywords: | Bone Marrow Mesenchymal Stem Cell Transplantation Alzheimer Disease Tissue Engineering |
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