| 碱性成纤维细胞生长因子及胰岛素样生长因子影响骨髓间充质干细胞增殖及胶原合成 |
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| 引用本文： | 徐海龙,丁 越,谢 洪,孙晓菊,谢慧心. 碱性成纤维细胞生长因子及胰岛素样生长因子影响骨髓间充质干细胞增殖及胶原合成[J]. 中国组织工程研究, 2016, 20(6): 891-897. DOI: 10.3969/j.issn.2095-4344.2016.06.019 |
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| 作者姓名： | 徐海龙 丁 越 谢 洪 孙晓菊 谢慧心 |
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| 作者单位： | 辽宁省人民医院，辽宁省沈阳市 110016；中国医科大学研究生院，辽宁省沈阳市 110001 |
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| 基金项目： | 辽宁省科学技术计划项目(2013225021)，“恶性肿瘤转化医学研究中心及协同研究网络建设” |
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| 摘 要： | ![]()
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| 关 键 词： | 干细胞 骨髓干细胞 碱性成纤维细胞生长因子 胰岛素样生长因子1 骨髓间充质干细胞 细胞增殖 Ⅰ型胶原 Ⅲ型胶原 |
| 收稿时间： | 2015-12-19 |
Effect of basic fibroblast growth factor and insulin-like growth factor-1 on proliferation and collagen synthesis of bone marrow mesenchymal stem cells |
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| Xu Hai-long,Ding Yue,Xie Hong,Sun Xiao-ju,Xie Hui-xin. Effect of basic fibroblast growth factor and insulin-like growth factor-1 on proliferation and collagen synthesis of bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(6): 891-897. DOI: 10.3969/j.issn.2095-4344.2016.06.019 |
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| Authors: | Xu Hai-long Ding Yue Xie Hong Sun Xiao-ju Xie Hui-xin |
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| Affiliation: | the People’s Hospital of Liaoning Province, Shenyang 110016, Liaoning Province, China; Graduate School of China Medical University, Shenyang 110001, Liaoning Province, China |
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| Abstract: | ![]()
BACKGROUND: How to control the orderly formation of collage in skin repair and scarring process is worthy of attention.OBJECTIVE: To investigate the effect of basic fibroblast growth factor (bFGF) combined with insulin-like growth factor 1 (IGF-1) on the proliferation and collagen synthesis of rat bone marrow mesenchymal stem cells in vitro.METHODS: Rat bone marrow mesenchymal stem cells were isolated and cultured to induce adipogenic differentiation assessed by oil red O staining and osteogenic differentiation identified by alizarin red staining in vitro. Passage 3 cells were cultured in the medium containing bFGF, IGF-1, combination of them or the control fluid, respectively. MTT assay was used to detect cell proliferation at 12, 24, 48, 72 and 96 hours of culture. The expression of type I collagen and type III collagen were detected by RT-PCR and western blot after 10 days of incubation.RESULTS AND CONCLUSION: Compared with the control group, bFGF or IGF-1 alone significantly promoted the proliferation of bone marrow mesenchymal stem cells, and inhibited the expression of type I collagen and type III collagen. After combined use of bFGF and IGF-1, the proliferation of bone marrow mesenchymal stem cells was improved more significantly, and the expression of type I collagen and type III collagen returned to normal levels. These findings indicate that the combination of IGF-1 and bFGF can promote proliferation of bone marrow mesenchymal stem cells and restrain the expression of type I collagen and type III collagen, which may be helpful for control and repair of scar formation during wound healing. |
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