| 阿伐他汀激活wnt/β-catenin信号通路促进骨质疏松模型大鼠移植物的骨整合 |
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| 引用本文： | 梁耀中,陈 舒,杨裕豪,蓝春海,张国威,纪志盛,林宏生. 阿伐他汀激活wnt/β-catenin信号通路促进骨质疏松模型大鼠移植物的骨整合[J]. 中国组织工程研究, 2016, 20(20): 2940-2948. DOI: 10.3969/j.issn.2095-4344.2016.20.008 |
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| 作者姓名： | 梁耀中 陈 舒 杨裕豪 蓝春海 张国威 纪志盛 林宏生 |
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| 作者单位： | 暨南大学附属第一医院，1骨科，2妇产科，广东省广州市 510630 |
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| 基金项目： | 广东省自然科学基金(2014A030313357) |
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| 摘 要： | BACKGROUND: Atorvastatin has been shown to reduce bone loss and fracture, but its effects on implant osseointegration remain unknown. OBJECTIVE: To investigate the effects of atorvastatin on implant osseointegration in osteoporotic rats and the underlying mechanisms. METHODS: Forty-eight Sprague-Dawley rats were randomized into sham-surgery, ovariectomy, and atorvastatin (10 and 20 mg/kg per day) treatment groups, respectively. All rats received ovariectomy and implant surgery except those in the sham-surgery group. Bone mineral density of the lumbar vertebra, osseointegration ratio and pull-out strength of implants were measured after 12-week treatment. Levels of bone formation and resorption markers in osteoblasts treated with atorvastatin were determined by ELISA. Wnt pathway-related gene expression was detected by RT-PCR. RESULTS AND CONCLUSION: Bone mineral density, osseointegration ratio and pull-out strength of implants were significantly increased in 20 mg/kg per day of atorvastatin treatment group compared with ovariectomy group (P < 0.05). Levels of alkaline phosphatase, osteocalcin and osteoprotegerin were significantly increased in osteoblasts treated with atorvastatin in vitro (P < 0 .05), and the level of osteoclast differentiation factor RANKL was significantly inhibited (P < 0.05). Meanwhile, atorvastatin significantly promoted the mRNA expression of low-density lipoprotein associated protein 5 and β-catenin, and inhibited the mRNA expression of dickkopf Wnt signal pathway inhibitor 1 and sclerostin. Our results suggest that atorvastatin promotes implant osseointegration in osteoporotic rats by activating Wnt/β-catenin signal pathway.中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程
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| 关 键 词： | 组织构建 骨组织工程 阿伐他汀 卵巢切除术 移植性骨整合 骨质疏松症 高血脂症 碱性磷酸酶 骨保护素 骨钙蛋白 破骨分化因子 Wnt信号通路 广东省自然科学基金 |
| 收稿时间： | 2016-04-06 |
Atorvastatin promotes implant osseointegration via the activation of Wnt/β-catenin signal pathway in osteoporotic rats |
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| Liang Yao-zhong,Chen Shu,Yang Yu-hao,Lan Chun-hai,Zhang Guo-wei,Ji Zhi-sheng. Atorvastatin promotes implant osseointegration via the activation of Wnt/β-catenin signal pathway in osteoporotic rats[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(20): 2940-2948. DOI: 10.3969/j.issn.2095-4344.2016.20.008 |
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| Authors: | Liang Yao-zhong Chen Shu Yang Yu-hao Lan Chun-hai Zhang Guo-wei Ji Zhi-sheng |
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| Affiliation: | 1 Department of Orthopedics, the First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, China
2 Department of Gynaecology and Obstetrics, the First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, China |
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| Abstract: | |
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| Keywords: | Osteoporosis Ovariectomy Bone Transplantation Osseointegration Tissue Engineering |
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