| 超微超顺磁性纳米氧化铁标记犬口腔黏膜上皮细胞与磁共振成像实验 |
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| 引用本文： | 周术奎,姚婷婷,张楷乐,邹青松,傅 强. 超微超顺磁性纳米氧化铁标记犬口腔黏膜上皮细胞与磁共振成像实验[J]. 中国组织工程研究, 2016, 20(52): 7796-7802. DOI: 10.3969/j.issn.2095-4344.2016.52.006 |
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| 作者姓名： | 周术奎 姚婷婷 张楷乐 邹青松 傅 强 |
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| 作者单位： | 上海交通大学附属第六人民医院，泌尿外科，放射科，上海市 200233 |
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| 基金项目： | 国家自然科学基金资助项目(81270780)；上海市第六人民医院院内课题(院内-1711) |
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| 摘 要： | ![]()
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| 关 键 词： | 生物材料 纳米材料 超微超顺磁性纳米氧化铁 口腔上皮细胞 磁共振成像 组织工程 国家自然科学基金 |
| 收稿时间： | 2016-09-19 |
Magnetic resonance imaging of canineoral epithelial cells labeled with ultrasmall superparamagnetic iron oxide |
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| Zhou Shu-kui,Yao Ting-ting,Zhang Kai-le,Zou Qing-song,Fu Qiang. Magnetic resonance imaging of canineoral epithelial cells labeled with ultrasmall superparamagnetic iron oxide[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(52): 7796-7802. DOI: 10.3969/j.issn.2095-4344.2016.52.006 |
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| Authors: | Zhou Shu-kui Yao Ting-ting Zhang Kai-le Zou Qing-song Fu Qiang |
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| Affiliation: | Department of Urinary Surgery, Department of Radiology, Affiliated Sixth People’s Hospital, Shanghai Jiao Tong University, Shanghai 200233, China |
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| Abstract: | ![]()
BACKGROUND: Epithelial cells are commonly used as the seed cell in tissue engineering; however, there is still a lack of an effective in vivo noninvasive trace technology. OBJECTIVE: To investigate the feasibility of labeling canine oral epithelial cells with ultrasmall superparamagnetie iron oxide (USPIO) and magnetic resonance imaging (MRI) in vitro. METHODS: Oral epithelial cells from beagles were primary cultured, and then labeled by 0.75 mg/L poly-L-lysine combined with USPIO (0, 5, 10, 25, 50 and 100 mg/L), respectively. To determine the optimal dosage, the intracellular iron expression was identified by Prussian blue staining, and the cell viability in different groups was detected by cell counting kit-8. Finally, 2×105 labeled cells were suspended with 1 mL PBS buffer, and were screened using 3.0 T MR on T2*WI sequences in vitro. RESULTS AND CONCLUSION: USPIO prepared with 0.75 mg/L poly-L-lysine could successfully label dog oral epithelial cells. Prussian blue staining showed intracellular blue spots, and the intracellular blue spots became more with the concentration increasing and saturated at the concentration of 25 mg/L. Cell counting kit-8 indicated that the cell viability did not change when the concentration < 25 mg/L. Among the T2*WI sequences, the MRI signal intensity decreased with the concentration increasing. In conclusion, canine oral epithelial cells can be effectively labeled with USPIO making no impact on cell viability when the concentration < 25 mg/L, and MRI can be used to track these labeled cells in vitro． |
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| Keywords: | Iron Compounds Nanostructures Magnetic Resonance Imaging Epithelial Cells Tissue Engineering |
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