| 巨噬细胞中脂多糖诱导微小RNA-155表达与地塞米松的抑制 |
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| 引用本文： | 王中华,王首红,吴 岩,李 宙,廖小龙,覃铁和. 巨噬细胞中脂多糖诱导微小RNA-155表达与地塞米松的抑制[J]. 中国组织工程研究, 2016, 20(11): 1591-1596. DOI: 10.3969/j.issn.2095-4344.2016.11.011 |
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| 作者姓名： | 王中华 王首红 吴 岩 李 宙 廖小龙 覃铁和 |
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| 作者单位： | 广东省人民医院(广东省医学科学院)，广东省老年医学研究所重症医学科，广东省广州市 510080 |
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| 摘 要： |
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| 关 键 词： | 组织构建 组织工程 微小RNA-155 巨噬细胞 地塞米松 脂多糖 炎症 |
| 收稿时间： | 2016-02-10 |
Dexamethasone inhibits the expression of microRNA-155 in macrophages induced by lipopolysaccharide |
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| Wang Zhong-hua,Wang Shou-hong,Wu Yan,Li Zhou,Liao Xiao-long,Qin Tie-he. Dexamethasone inhibits the expression of microRNA-155 in macrophages induced by lipopolysaccharide[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(11): 1591-1596. DOI: 10.3969/j.issn.2095-4344.2016.11.011 |
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| Authors: | Wang Zhong-hua Wang Shou-hong Wu Yan Li Zhou Liao Xiao-long Qin Tie-he |
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| Affiliation: | Guangdong Provincial Institute of Geriatrics, Guangdong General Hospital (Guangdong Academy of Medical Science), Guangzhou 510080, Guangdong Province, China |
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| Abstract: | BACKGROUND: It is unclear about dexamethasone effect on the regulation of microRNA-155 expression in macrophages.OBJCTIVE: To explore whether dexamethasone can regulate the expression of microRNA-155 in macrophages.METHODS: (1) Lipopolysaccharide stimulation of mouse macrophages: mouse macrophage cell lines, Raw264.7 cells, were cultured in vitro and stimulated by lipopolysaccharide. Cultured cells were collected at 0, 0.5, 2, 6 hours after culture to detect the dynamical expression of microRNA-155. (2) Dexamethasone intervention for macrophages: Macrophages were divided into four groups: control group treated with phosphate buffer; lipopolysaccharide group stimulated by lipopolysaccharide; combined group given intervention with dexamethasone and lipopolysaccharide; dexamethasone group cultured with dexamethasone. At 6 hours after culture, cell supernatant was collected to detect the expression of tumor necrosis factor α and interleukin-6 using ELISA method. Real-time fluorescence quantitative PCR was used to detect the expression of microRNA-155 in the Raw264.7 macrophages.RESULTS AND CONCLUSION: Lipopolysaccharide significantly increased the expression of tumor necrosis factor α, interleukin-6 and microRNA-155 after 6 hours of culture (P < 0.05). Combined use of dexamethasone and lipopolysaccharide slightly increased the expression of tumor necrosis factor α, interleukin-6 and microRNA-155 (P < 0.05). Dexamethasone alone had no influence on the expression of tumor necrosis factor α and interleukin-6, but significantly decreased the expression of microRNA-155 (P < 0.05). These findings indicate that dexamethasone can inhibit the expression of microRNA-155 in the macrophages induced by lipopolysaccharide. |
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