| 骨髓间充质干细胞移植治疗系统性红斑狼疮 |
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| 引用本文： | 王治国,刘学明,佟胜全,石哲群,饶 莉. 骨髓间充质干细胞移植治疗系统性红斑狼疮[J]. 中国组织工程研究, 2016, 20(10): 1461-1467. DOI: 10.3969/j.issn.2095-4344.2016.10.013 |
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| 作者姓名： | 王治国 刘学明 佟胜全 石哲群 饶 莉 |
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| 作者单位： | 河北医科大学附属唐山工人医院，风湿免疫科，神经内科，河北省唐山市 063000 |
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| 基金项目： | 河北省医学科学研究重点课题计划(20150948) |
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| 摘 要： |
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| 关 键 词： | 干细胞 移植 系统性红斑狼疮 MRL/LPR小鼠 骨髓间充质干细胞 干细胞移植 CD4+CD25+T细胞水平 血清ds-DNA抗体 |
| 收稿时间： | 2016-01-17 |
Bone marrow mesenchymal stem cell transplantation for the treatment of systemic lupus erythematosus |
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| Wang Zhi-guo,Liu Xue-ming,Tong Sheng-quan,Shi Zhe-qun,Rao Li. Bone marrow mesenchymal stem cell transplantation for the treatment of systemic lupus erythematosus[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(10): 1461-1467. DOI: 10.3969/j.issn.2095-4344.2016.10.013 |
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| Authors: | Wang Zhi-guo Liu Xue-ming Tong Sheng-quan Shi Zhe-qun Rao Li |
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| Affiliation: | Department of Rheumatology, Department of Neurology, Tangshan Gongren Hospital, Hebei Medical University, Tangshan 063000, Hebei Province, China |
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| Abstract: | BACKGROUND: The non-specific immune suppression method is generally used for treatment of systemic lupus erythematosus, but poor prognosis, such as infection and high recurrence rate, exists. OBJECTIVE: To evaluate the therapeutic effect of bone marrow mesenchymal stem cell transplantation on systemic lupus erythematosus in mice.METHODS: Sixteen mice with systemic lupus erythematosus were equivalently randomized into control and experimental groups, or then subjected to passage 3 bone marrow mesenchymal stem cell transplantation or the equal volume of normal saline via the tail vein, respectively. Mouse urine samples were collected to detect urine protein levels by Bradford method. Blood samples from the tip of the mouse tail were extracted to detect serum anti-ds-DNS antibody concentration by radioimmunoassay. Mouse kidney tissues were taken and observed pathohistologically through hematoxylin-eosin staining and immunohistochemistry staining under microscope. Flow cytometry was used to detect the expression of CD4+CD25+T cells in the inner canthus blood, fresh spleen and thymus.RESULTS AND CONCLUSION: Within 10 weeks after cell transplantation, the urine protein levels in the two groups were gradually increased, and the rising velocity was higher in the control group than in the experimental group. From the 4th to 10th week, the urine protein levels in the experimental group were significantly lower than those in the control group (P < 0.05). In the control group, lymphocyte infiltration was visible in the kidney tissues with a few of plasmocytes, and pathological findings showed the mice presented with interstitial nephritis; in the experimental group, the mice had no pathological changes in the kidney. In the two groups, immune complexes were found in the mesangial area, which showed a patch-like distribution in the control group and a punctate distribution in the experimental group; the relative proportion of the occupied area in the experimental group was significantly lower than that in the control group. The expression level of CD4+CD25+T cells in the blood and thymus were significantly higher in the experimental group than the control group (P < 0.05), and the expression level of CD4+CD25+T cells in the spleen was slightly higher in the experimental group than the control group with no significant difference (P > 0.05). The serum anti-ds-DNA antibody concentration in the experimental group was significantly lower than that in the control group (P < 0.05). Taken together, bone marrow mesenchymal stem cell transplantation can improve the pathological damage in systemic lupus erythematosus mice, and has a certain therapeutic effect on systemic lupus erythematosus. |
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