| 五类栽培白芷与兴安白芷的挥发油成分及其化学亲缘关系 |
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| 引用本文： | 田恩伟,成欣,刘银榕,陈建玲,晁志. 五类栽培白芷与兴安白芷的挥发油成分及其化学亲缘关系[J]. 南方医科大学学报, 2022, 42(3): 360-366. DOI: 10.12122/j.issn.1673-4254.2022.03.07 |
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| 作者姓名： | 田恩伟 成欣 刘银榕 陈建玲 晁志 |
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| 作者单位： | 南方医科大学中医药学院，广东 广州 510515；南方医科大学珠江医院药剂科，广东 广州 510280；广东省中药制剂重点实验室，广东 广州 510515；广东省中药制剂技术工程实验室，广东 广州 510515 |
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| 摘 要： | 目的 测定5类栽培白芷及野生兴安白芷（根）的挥发油成分及含量并分析各类白芷间的化学亲缘关系。方法 水蒸气蒸馏法提取6类白芷挥发油，采用GC-MS法对其组分分离及鉴定，采用面积归一化法计算各组分相对含量，并对挥发油成分进行聚类分析和主成分分析。结果 5类栽培白芷（川白芷、杭白芷、祁白芷、禹白芷、毫白芷）和野生兴安白芷共鉴定出81种化合物，分别鉴定出27、34、24、32、28、34种。主要为烷烃类、烯烃类、酯类、有机酸类、醇类等五大类化合物。壬基环丙烷、环十二烷和正十六酸分别为各类白芷共有成分中相对含量最高的挥发油成分。化学聚类结果显示：兴安白芷与5类栽培白芷形成2个分支，栽培白芷又可分为2小类，即川白芷、禹白芷、杭白芷聚为1小类，祁白芷和亳白芷聚为另1小类。主成分分析结果与聚类结果基本一致。结论 六类白芷的挥发油组份及其含量存在一定差异，壬基环丙烷、环十二烷和正十六酸在各类白芷中含量较高。六类白芷总体上分为野生与栽培2大类。
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| 关 键 词： | 白芷；挥发油；化学亲缘关系；聚类分析；气相色谱-质谱联用 |
Analysis of volatile constituents and chemical relationship of cultivated and wild Angelica dahurica |
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| TIAN Enwei,CHENG Xin,LIU Yinrong,CHEN Jianling,CHAO Zhi. Analysis of volatile constituents and chemical relationship of cultivated and wild Angelica dahurica[J]. Journal of Southern Medical University, 2022, 42(3): 360-366. DOI: 10.12122/j.issn.1673-4254.2022.03.07 |
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| Authors: | TIAN Enwei CHENG Xin LIU Yinrong CHEN Jianling CHAO Zhi |
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| Affiliation: | School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China; Department of Pharmacy, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China; Guangdong Provincial Key Laboratory of Chinese Medicine Pharmaceutics, Guangzhou 510515, China; Guangdong Provincial Engineering Laboratory of Chinese Medicine Preparation Technology, Guangzhou 510515, China |
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| Abstract: | Objective To determine the volatile constituents and their contents in the roots of 5 cultivated Angelica dahurica and one wild A. dahurica and analyze the chemical relationship among the plants of A. dahurica. Methods The essential oil was extracted from the roots of 5 cultivated plants of Angelica dahurica and one wild A. dahurica by water steam distillation. Gas chromatography-mass spectrometry (GC-MS) was used to separate and identify all the volatile oil components in the extracts, and their relative contents were calculated with area normalization method. We also conducted clustering analysis and principal component analysis of the volatile oil components. Results We identified a total of 81 compounds from the roots of the 6 plants of Angelica dahurica, including 27 in Chuanbaizhi (Angelica dahurica cv. 'Hangbaizhi'), 34 in Hangbaizhi (Angelica dahurica cv. 'Hangbaizhi'), 24 in Qibaizhi (Angelica dahurica cv. 'Qibaizhi'), 32 in Yubaizhi (Angelica dahurica cv.'Qibaizhi'), 28 in Bobahizhi (Angelica dahurica cv.'Qibaizhi'), and 34 in Xinganbaizhi (Angelica dahuirca). These compounds included, in the order of their relative contents (from high to low), alkanes, olefins, esters, organic acids and alcohols. Among the common components found in the roots of all the plants of A. dahurica, nonylcyclopropane, cyclododecane and hexadecanoic acid were identified as the volatile oil components that showed the highest relative contents. Clustering analysis of the volatile oil components showed that wild Angelica dahurica (Xing'anbaizhi) and the 5 cultivated Angelica dahurica (Chuanbaizhi, Hangbaizhi, Qibaizhi, Yubaizhi, Bobaizhi) could be divided into two groups, and the cultivated Angelica dahurica could be divided into two subgroups: Chuanbaizhi, Yubaizhi and Hangbahizhi were clustered in one subgroup, and Qibaizhi and Bobaizhi in another. The results of principal component analysis was consistent with those of clustering analysis. Conclusion The main volatile oil components and their contents vary among the 6 plants of A. dahurica. Nonylcyclopropane, cyclododecane and hexadecanoic acid are the most abundant volatile oil components in all the plants of A. dahurica, which can be divided into two clusters. |
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| Keywords: | Angelica dahurica volatile oil chemical relationship clustering analysis gas chromatography-mass spectrometry, |
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