Subunit stoichiometry and juxtaposition of the photosynthetic coupling factor 1: Immunoelectron microscopy using monoclonal antibodies |
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| Authors: | Tiedge H Lünsdorf H Schäfer G Schairer H U |
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| Affiliation: | Institut für Biochemie, Medizinische Hochschule Lübeck, Ratzeburger Allee 160, D-2400 Lübeck, Federal Republic of Germany. |
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| Abstract: | Monoclonal antibodies specific to the α subunits of the photosynthetic coupling factor 1 (CF1) were used as marker molecules in an electron microscopic analysis of the subunit organization of this enzyme. Immune complexes were obtained by incubation of CF1 with saturating amounts of anti-α-subunit IgG, isolated by gel filtration, and visualized by electron microscopy. The maximum number of antibodies bound to a CF1 molecule was three, the angle defined by a neighboring pair of antibodies characteristically being 120°. These results are interpreted as direct evidence for the presence of three α subunits in the CF1 complex, the relative orientation of them being described by 3-fold rotary symmetry. Our observations thus favor an overall subunit stoichiometry of α3β3γδε. |
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