| 侧脑室内连续注射神经肽S对神经病理性疼痛大鼠疼痛及焦虑行为的影响 |
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| 引用本文： | 金旭,曾敏,熊蔚,罗芳. 侧脑室内连续注射神经肽S对神经病理性疼痛大鼠疼痛及焦虑行为的影响[J]. 中国康复理论与实践, 2014, 20(7): 621-625 |
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| 作者姓名： | 金旭 曾敏 熊蔚 罗芳 |
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| 作者单位： | 首都医科大学附属北京天坛医院麻醉科，北京市100050。 |
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| 摘 要： | 目的研究侧脑室内连续注射神经肽S对神经病理性疼痛大鼠疼痛行为及焦虑行为的影响，探讨神经肽S对疼痛和焦虑影响的作用机制。方法40 只雄性Sprague-Dawley 大鼠被随机分为假手术对照组(Sham-Veh 组)、假手术神经肽S 组(Sham-NPS组)、慢性压迫性神经损伤组(CCI 组)、神经肽S 低剂量组(NPSl 组，0.1 nmol/L)和神经肽S 高剂量组(NPSh 组，1 nmol/L)，每组8只。制备CCI动物模型，造模成功后第7 天左侧侧脑室置入微透析用导针，自术后第8 天起每日侧脑室注射相应药物，在术后第14天检测机械痛缩足阈值和热辐射缩足潜伏期，并通过高架十字迷宫检测焦虑行为的变化。采用免疫组织荧光技术检测左侧杏仁核区神经肽S受体(NPSR)的表达。结果与Sham-Veh 组比较，CCI组大鼠术后14 d 出现明显疼痛和焦虑行为(P<0.01)，NPSh组明显缓解(P<0.01)，NPSl组与CCI组比较无显著性差异(P>0.05)。免疫组织荧光结果显示，与CCI组相比，NPSh组杏仁核区NPSR表达明显增加。结论神经肽S可以剂量依赖性地逆转CCI大鼠的疼痛和焦虑行为，其机制可能与杏仁核NPSR上调有关。
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| 关 键 词： | 神经病理性疼痛 神经肽S 焦虑 侧脑室注射 杏仁核 大鼠 |
| 收稿时间： | 2014-03-14 |
Effect of Intracerebroventricular Injection of Neuropeptide S on Pain and Anxiety Behaviors in Rats with Neuropathic Pain |
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| JIN Xu,ZENG Min,XIONG Wei,et al.. Effect of Intracerebroventricular Injection of Neuropeptide S on Pain and Anxiety Behaviors in Rats with Neuropathic Pain[J]. Chinese Journal of Rehabilitation Theory and Practice, 2014, 20(7): 621-625 |
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| Authors: | JIN Xu ZENG Min XIONG Wei et al. |
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| Affiliation: | Department of Anesthesia and Pain Therapy, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China |
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| Abstract: | Objective To investigate the effects of intracerebroventricular injection of neuropeptide S (NPS) on pain and anxiety behaviors in rats with neuropathic pain, and explore the possible mechanism. Methods 40 male Sprague-Dawley rats were randomly divided into Sham-Vehicle group, Sham-NPS group, chronic constriction injury (CCI) group, Neuropeptide S low-dose group (NPSl group, 0.1 nmol/L) and Neuropeptide S high-dose group (NPSh group, 1 nmol/L), with 8 rats in each group. Pain-related behaviors, anxiety-related behaviours,and expression of NPS receptor (NPSR) in the left amygdaloid nucleus were measured on the 14th day via intracerebroventricular injection.Results Compared with the Sham-Vehicle group, the CCI group demonstrated significant pain and anxiety behaviors 14 days after operation (P<0.01), the NPSh group significantly relieved (P<0.01). And there was no significant difference between the NPSl group and the CCI group (P>0.05). Compared with the CCI group, NPSR expression in amygdaloid nucleus increased in the NPSh group. Conclusion NPS can dose-dependently relieve the pain and anxiety behaviors in CCI rats, which may be related with the increase of NPSR in amygdaloid nucleus. |
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| Keywords: | neuropathic pain neuropeptide S anxiety intracerebroventricular injection amygdaloid nucleus rats |
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