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| 不同强度运动对大鼠关节软骨缺损早期修复和MMP-3、TIMP-1表达的影响 | |
| 引用本文： | 董 福,宋锦旗,罗吉伟,赵培冉,倪国新,余 斌. 不同强度运动对大鼠关节软骨缺损早期修复和MMP-3、TIMP-1表达的影响[J]. 南方医科大学学报, 2014, 34(1): 103 |
| 作者姓名： | 董 福 宋锦旗 罗吉伟 赵培冉 倪国新 余 斌 |
| 摘 要： | 目的比较不同强度运动对大鼠髌股关节软骨缺损早期修复及血清基质金属蛋白酶-3（MMP-3）、基质金属蛋白酶抑制 剂-1（TIMP-1）表达的影响。方法将24 只雄性SD大鼠建立双侧髌股关节全厚软骨缺损模型，后随机分为4 组：安静对照组 （SED）、低强度运动组（LIR）、中强度运动组（MIR）、高强度运动组（HIR），后3组施行跑台运动6周。于实验前、后分别用ELISA 法测定血清MMP-3、TIMP-1浓度，并计算实验后TIMP-1/MMP-3浓度比值。实验后切开关节行大体观察评分，软骨缺损处组 织分别予蕃红O-固绿、甲苯胺蓝染色行组织学观察、O’Driscoll组织评分。结果（1）组织学切片显示：SED组软骨缺损处类透 明软骨填充，LIR组、MIR组均为纤维组织覆盖，HIR组软骨下骨破坏；大体评分与O’Driscoll组织评分：SED组均高于3运动组 （P<0.05），HIR 组最低；（2）MMP-3、TIMP-1 浓度：实验前4 组MMP-3、TIMP-1 浓度差异无统计学意义（P>0.05）；实验后4 组 MMP-3、TIMP-1 浓度均明显高于实验前（P<0.05），组间差异有统计学意义（P<0.05），运动组MMP-3 浓度均高于SED组（P< 0.05）；（3）TIMP-1/MMP-3浓度比值：SED组明显高于3运动组（P<0.05），HIR组最低，LIR与MRI组居中。结论中、低强度运动 均抑制大鼠髌股关节全厚软骨缺损的早期修复，高强度运动破坏软骨下骨，MMP-3、TIMP-1表达与运动相关，TIMP-1/MMP-3 值与软骨修复的程度一致。 |
Effects of exercise of different intensity on early repair of full-thickness articularcartilage defects and expressions of MMP-3 and TIMP-1 in rats |
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| Abstract: | Objective To evaluate the effects of treadmill running exercise of different intensity on early repair of full-thickness defects on the patellofemoral articular surface and the changes in the expressions of matrix metalloproteinase-3 (MMP-3) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in SD rats. Methods Twenty-four male SD rats with full-thickness defects on the patellofemoral articular surface were randomly assigned into sedentary control (SED) group and low-, moderate- and high-intensity running groups (LIR, MIR, and HIR groups, respectively). The running groups were trained on treadmill for 6 consecutive weeks. Blood samples were collected to detect serum MMP-3 and TIMP-1 levels using ELISA before and after the experiment, and the femoral trochleas were collected to assess tissue repair by gross appearance scoring and O’Driscoll histological scoring with Safranine O-Fast Green staining and Toluidine blue staining. Results In rats in SED group, the defect was filled with hyaline articular cartilage-like tissues, as compared to fibrous tissues in LIR and MIR groups and subchondral bone damage in HIR group. The SED group scored the highest and HIR group the lowest among the 4 groups in gross appearance scoring and O’Driscoll histological scoring. No significant differences were found in MMP-3 or TIMP-1 levels among the groups before training (P>0.05), but after 6 weeks of training, serum MMP-3 and TIMP-1 levels differed significantly among the 4 groups (P<0.05), and all the 3 running groups had a significantly higher MMP-3 level than the control group (P<0.05). After the 6-week training, TIMP-1/MMP-3 ratio was significantly higher in SED group than in the 3 running groups, and was the lowest in HIR group. Conclusion Both low- and moderate-intensity exercise failed to promote resurfacing of full-thickness cartilage defects on the patellofemoral articular surface in rats, and high-intensity exercise even induces subchondral bone damage. The expression of MMP-3 and TIMP-1 is related to exercise, and the TIMP-1/MMP-3 ratio reflects the extent of tissue repair. |
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