| 沉默MTA1抑制肝癌细胞黏附、迁移和侵袭能力的实验研究 |
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| 引用本文： | 赵鹏伟,黄建钊,柳 严,刘 延,刘江伟,张佳伟. 沉默MTA1抑制肝癌细胞黏附、迁移和侵袭能力的实验研究[J]. 现代肿瘤医学, 2020, 0(2): 214-219. DOI: 10.3969/j.issn.1672-4992.2020.02.008 |
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| 作者姓名： | 赵鹏伟 黄建钊 柳 严 刘 延 刘江伟 张佳伟 |
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| 作者单位： | 贵州省人民医院肝胆外科，贵州 贵阳 550002 |
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| 摘 要： | 目的:研究沉默转移相关基因1(MTA1)对肝癌细胞黏附、迁移和侵袭能力的影响。方法:在肝癌细胞中转染MTA1 siRNA、siRNA control，同时以不做转染的细胞作为对照，qRT-PCR和Western blot分别检测细胞中MTA1的表达水平，MTT检测细胞增殖，细胞黏附试验检测细胞黏附能力，Transwell小室检测细胞侵袭和迁移能力，Western blot检测细胞中基质金属蛋白酶-2（MMP-2）、上皮钙黏附素(E-cadherin)、基质金属蛋白酶-9（MMP-9）、神经型钙黏素(N-cadherin)蛋白水平,明胶酶谱实验检测MMP-2和MMP-9活性。结果:细胞中转染MTA1 siRNA能够下调肝癌细胞中MTA1 mRNA和蛋白水平，转染siRNA control对细胞中MTA1 mRNA和蛋白水平没有影响。沉默MTA1后的肝癌细胞存活率和黏附率降低，侵袭细胞数目减少，与没有转染的细胞比较，差异有统计学意义（P＜0.05）。沉默MTA1后的肝癌细胞中MMP-2、MMP-9蛋白水平及活性降低，细胞中E-cadherin蛋白水平升高，N-cadherin蛋白水平降低，与没有转染的细胞比较，差异有统计学意义（P＜0.05）。结论:沉默MTA1抑制肝癌细胞黏附、迁移和侵袭能力，作用机制与抑制细胞分泌MMP-2、MMP-9及抑制细胞EMT有关。
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| 关 键 词： | 肝癌细胞 黏附 迁移 侵袭 MTA1 |
Silencing MTA1 inhibits the adhesion,migration and invasion of hepatocellular carcinoma cells |
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| Zhao Pengwei,Huang Jianzhao,Liu Yan,Liu Yan,Liu Jiangwei,Zhang Jiawei. Silencing MTA1 inhibits the adhesion,migration and invasion of hepatocellular carcinoma cells[J]. Journal of Modern Oncology, 2020, 0(2): 214-219. DOI: 10.3969/j.issn.1672-4992.2020.02.008 |
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| Authors: | Zhao Pengwei Huang Jianzhao Liu Yan Liu Yan Liu Jiangwei Zhang Jiawei |
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| Affiliation: | Department of Hepatobiliary Surgery,Guizhou People's Hospital,Guizhou Guiyang 550002,China. |
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| Abstract: | Objective:To study the effect of silencing MTA1 on the adhesion,migration and invasion on hepatocellular carcinoma cells.Methods:MTA1 siRNA and siRNA control were transfected into hepatoma cells,at the same time,the cells without transfection were used as control.qRT-PCR and Western blot were used to detect the expression level of MTA1.MTT detection of cell proliferation,cell adhesion test was used to detect cell adhesion.Transwell was used to detect cell invasiveness and migration.Western blot was used to detect the levels of MMP-2,MMP-9,E-cadherin and N-cadherin protein in cells.Gelatinase zymography was used to detect the activity of MMP-2 and MMP-9.Results:Transfection of MTA1 siRNA in cells could downregulate the level of MTA1 mRNA and protein in hepatoma cells.Transfection of siRNA control had no effect on MTA1 mRNA and protein level in cells.After silencing MTA1,the survival rate and adhesion rate of hepatoma cells were decreased.The number of invasive cells was reduced.Compared with cells without transfection,the difference was statistically significant (P＜0.05).After silencing MTA1,the levels of MMP-2,MMP-9 protein and activity were decreased,at the same time,the level of E-cadherin protein was increased,and the level of N-cadherin protein was reduced.Compared with cells without transfection,the difference was statistically significant (P＜0.05).Conclusion:Silencing MTA1 inhibits the adhesion,migration and invasion of hepatocellular carcinoma cells.The mechanism is related to the inhibition of cell secretion of MMP-2,MMP-9 and inhibition of EMT. |
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| Keywords: | hepatoma cells adhesion migration invasion MTA1 |
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