| miR-181a表达水平与ER阳性乳腺癌对内分泌治疗敏感性的关系 |
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| 引用本文： | 闫顺朝1,焦 昕2,李 娜1,杜扬帆1. miR-181a表达水平与ER阳性乳腺癌对内分泌治疗敏感性的关系[J]. 现代肿瘤医学, 2020, 0(4): 573-577. DOI: 10.3969/j.issn.1672-4992.2020.04.011 |
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| 作者姓名： | 闫顺朝1 焦 昕2 李 娜1 杜扬帆1 |
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| 作者单位： | 1.中国医科大学附属盛京医院肿瘤科，辽宁 沈阳 110022；2.沈阳市胸科医院呼吸一科，辽宁 沈阳 110044 |
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| 基金项目： | National Natural Science Foundation of China（No.81302313）;国家自然科学基金项目（编号:81302313）；辽宁省自然科学基金指导计划（编号:20170540995）;沈阳市中青年科技创新人才支持计划项目（编号:RC170545） |
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| 摘 要： | 目的:研究miR-181a表达水平与雌激素受体（estrogen receptor,ER）阳性乳腺癌对内分泌治疗敏感性之间的关系。方法:采用梯度递增法诱导乳腺癌他莫昔芬耐药细胞株，利用集落形成实验检测细胞对他莫昔芬的敏感性。利用RT-PCR法检测miR-181a表达的情况，利用KM-plotter分析miR-181表达与乳腺癌患者预后之间的关系。结果:我们成功的诱导了MCF-7他莫昔芬继发耐药细胞株TAM-R。RT-PCR分析结果显示:他莫昔芬继发耐药的TAM-R细胞株及原发他莫昔芬耐药BT474细胞株中miR-181a的表达水平均显著高于对他莫昔芬敏感的MCF-7细胞株。生存分析结果显示:miR-181a的表达水平与ER阴性乳腺癌患者的总生存无关，但在ER阳性乳腺癌中miR-181a表达高的患者总生存明显差于miR-181a低表达者。进一步分析结果显示:在ER阳性但未接受内分泌治疗的乳腺癌患者中miR-181a表达与总生存无关，但在ER阳性且接受过内分泌治疗的乳腺癌患者中miR-181a高表达者总生存显著差于miR-181a低表达者。结论:miR-181a高表达ER阳性乳腺癌患者对内分泌治疗敏感性差，miR-181a可能是预测ER 阳性乳腺癌对内分泌治疗敏感性的指标。
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| 关 键 词： | miR-181a 乳腺癌 内分泌治疗 疗效 |
To explore the relationship between miR-181a and endocrine therapy efficiency of ER positive breast cancer |
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| Yan Shunchao1,Jiao Xin2,Li Na1,Du Yangfan1. To explore the relationship between miR-181a and endocrine therapy efficiency of ER positive breast cancer[J]. Journal of Modern Oncology, 2020, 0(4): 573-577. DOI: 10.3969/j.issn.1672-4992.2020.04.011 |
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| Authors: | Yan Shunchao1 Jiao Xin2 Li Na1 Du Yangfan1 |
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| Affiliation: | 1.Department of Oncology,Shengjing Hospital of China Medical University,Liaoning Shenyang 110022,China;2.Department of Respiratory Medicine,Shenyang Chest Hospital,Liaoning Shenyang 110044,China. |
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| Abstract: | Objective:To investigate the expression of miR-181a and endocrine-therapy sensitivity of estrogen receptor (ER) positive breast cancer.Methods:We constructed tamoxifen-resistant MCF-7 cell line using gradient incremental method.Cells proliferation was measured using colony formation assay.The expression of miR-181a was determined by RT-PCR.The prognostic value of miR-181a in breast cancer was analyzed using Kaplan-Meier Plotter.Results:We successfully constructed a cell line resistant to tamoxifen (TAM-R).RT-PCR results showed that the expression of miR-181a was higher in tamoxifen induced and primary resistance cell lines than tamoxifen sensitive cell line.High expression of miR-181a predicted significantly poorer overall survival (OS) in ER-positive but not ER-negative breast cancer.Further analysis showed that high expression of miR-181a was associated with poorer OS in endocrine-therapy treated ER-positive breast cancer but not in ER-positive breast cancer without endocrine-therapy treatment.Conclusion:High expression of miR-181a predicted less sensitive to endocrine therapy in ER positive breast cancer.miR-181a might be a promising predictor of the efficacy of endocrine therapy. |
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| Keywords: | miR-181a breast cancer endocrine therapy efficiency |
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