| 川陈皮素抑制胃癌SGC-7901细胞侵袭能力的机制探讨 |
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| 引用本文： | 杨雪竹1,张 浩2,崔西玉1,苏沛珠1,李兆滔1. 川陈皮素抑制胃癌SGC-7901细胞侵袭能力的机制探讨[J]. 现代肿瘤医学, 2020, 0(18): 3099-3104. DOI: 10.3969/j.issn.1672-4992.2020.18.001 |
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| 作者姓名： | 杨雪竹1 张 浩2 崔西玉1 苏沛珠1 李兆滔1 |
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| 作者单位： | 1.佛山市第一人民医院（中山大学附属佛山医院）消化内科，广东 佛山 528000;2.武汉大学人民医院肿瘤科，湖北 武汉 430061 |
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| 基金项目： | 广东省医学技术科学研究基金项目（编号:A2018291） |
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| 摘 要： | 目的:观察川陈皮素（nobiletin，Nob）对胃癌SGC-7901细胞侵袭能力的影响，并探讨其可能的作用机制。方法:不同浓度的川陈皮素（0、30、60、120 mg/L）体外处理SGC-7901细胞。CCK8法检测细胞增殖能力；Transwell小室法检测细胞侵袭能力的改变；免疫印迹法检测细胞上皮间质转化（epithelial-mesenchymal transition,EMT)标志物（E-cadherin、Vimentin、Fibronectin）蛋白、MMP-9、STAT3、p-STAT3蛋白表达的改变。结果:CCK8和Transwell小室法检测结果显示，与对照组（0 mg/L）比较，川陈皮素可显著抑制SGC-7901细胞的增殖和侵袭能力；免疫印迹结果显示，随着川陈皮素浓度的增加，SGC-7901细胞E-cadherin蛋白表达逐渐上调，同时Vimentin、Fibronectin蛋白的表达量降低，MMP-9蛋白表达下调,STAT3总量未发生变化，但p-STAT3表达逐渐降低。结论:川陈皮素可降低胃癌SGC-7901细胞侵袭能力，其机制可能为通过抑制STAT3通路，继而抑制EMT。
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| 关 键 词： | 川陈皮素 SGC-7901细胞 侵袭 上皮间质转化 STAT3信号通路 |
Study on the mechanism of nobiletin inhibiting the invasiveness of SGC-7901 gastric cancer cells |
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| Yang Xuezhu1,Zhang Hao2,Cui Xiyu1,Su Peizhu1,Li Zhaotao1. Study on the mechanism of nobiletin inhibiting the invasiveness of SGC-7901 gastric cancer cells[J]. Journal of Modern Oncology, 2020, 0(18): 3099-3104. DOI: 10.3969/j.issn.1672-4992.2020.18.001 |
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| Authors: | Yang Xuezhu1 Zhang Hao2 Cui Xiyu1 Su Peizhu1 Li Zhaotao1 |
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| Affiliation: | 1.Gastroenterology Department,Foshan First People's Hospital（Foshan Hospital Affiliated to Sun Yat-sen University），Guangdong Foshan 528000,China;2.Oncology Department,People's Hospital of Wuhan University,Hubei Wuhan 430061,China. |
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| Abstract: | Objective:To investigate the effect of nobiletin(Nob) on the cell invasiveness in SGC-7901 gastric cancer cells and explore the possible mechanisms.Methods:SGC-7901 cells were cultured in vitro.After treatment by nobiletin at different concentrations(0,30,60 and 120 mg/L) respectively,cell proliferation was detected by CCK8 assay and the invasion of SGC-7901 cells was tested by Transwell assay.Western blot was used for E-cadherin,Vimentin,Fibronectin,MMP-9,STAT3 and p-STAT3 protein analysis.Results:Results of CCK8 showed that cell proliferation was inhibited by nobiletin significantly and Transwell experiments revealed that the number of SGC-7901 cells treated by nobiletin passing through the basement membrane was less than that of the control group(P＜0.05),suggesting that nobiletin could inhibit the capacity of cell invasion.Nobiletin could promoted the expressions of E-cadherin,and suppressed the expressions of Vimentin,Fibronectin,MMP-9 and p-STAT3 in a dose-dependent manner.Conclusion:Nobiletin could inhibit the capacity of cell invasion,and the effects of antitumor may be associated with inhibition of the STAT3 signaling pathway,thus inhibiting of epithelial-to-mesenchymal transition in gastric cancer cell line SGC-7901 cells. |
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| Keywords: | nobiletin SGC-7901 cells invasion EMT STAT3 signaling pathway |
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