| MEMO1共结合IGF-IR介导胃癌细胞侵袭转移 |
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| 引用本文： | 王 智1,李贺明1,梁珊珊2,王若雨1. MEMO1共结合IGF-IR介导胃癌细胞侵袭转移[J]. 现代肿瘤医学, 2020, 0(2): 190-195. DOI: 10.3969/j.issn.1672-4992.2020.02.003 |
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| 作者姓名： | 王 智1 李贺明1 梁珊珊2 王若雨1 |
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| 作者单位： | 1.大连大学附属中山医院肿瘤科，辽宁 大连 116001；2.辽宁省乳腺及消化肿瘤分子标记物高通量筛选及靶向药物转化重点实验室，辽宁 大连 116001 |
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| 基金项目： | National Natural Science Foundation of China（No.81502124）;国家自然科学基金（编号:81502124）；2018年大连市科技创新基金计划（编号:2018J12SN063）；2019年大连市医学科学研究计划项目（编号:1911096） |
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| 摘 要： | 目的:探讨MEMO1（mediator of ErbB2-driven cell motility 1）调控IGF-IR（insulin-like growth factor-I receptor）介导胃癌侵袭转移的分子机制，为临床治疗提供潜在靶点。方法:选取五种不同分化程度胃癌细胞系（MKN-7、NCI-N87、MGC-803、BGC-823、MKN-74），Western blot检测MEMO1、IGF-IR和E-cad表达差异；Transwell比较五种胃癌细胞系迁移能力；选取MEMO1/IGF-IR高表达细胞系MGC-803，瞬时敲除MEMO1或IGF-IR基因，Western blot检测EMT标志物表达差异；IP实验检测MEMO1与IGF-IR间相互作用；TCGA数据库分析胃癌组织MEMO1和IGF-IR mRNA表达水平与患者预后的关系。结果:基础状态下五种胃癌细胞IGF-IR与MEMO1的表达呈显著正相关，并且两种蛋白的表达强度与胃癌细胞EMT相关；五种胃癌细胞迁移能力MKN-7
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| 关 键 词： | MEMO1 IGF-IR 胃癌 EMT |
The combination of MEMO1 with IGF-IR in the regulation of invasion and metastasis in gastric cancer cells |
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| Wang Zhi1,Li Heming1,Liang Shanshan2,Wang Ruoyu1. The combination of MEMO1 with IGF-IR in the regulation of invasion and metastasis in gastric cancer cells[J]. Journal of Modern Oncology, 2020, 0(2): 190-195. DOI: 10.3969/j.issn.1672-4992.2020.02.003 |
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| Authors: | Wang Zhi1 Li Heming1 Liang Shanshan2 Wang Ruoyu1 |
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| Affiliation: | 1.Department of Oncology,Affiliated Zhongshan Hospital of Dalian University,Liaoning Dalian 116001,China;2.The Key Laboratory of Biomarker High Throughput Screening and Target Translation of Breast and Gastrointestinal Tumor,Liaoning Dalian 116001,China. |
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| Abstract: | Objective:To investigate the molecular mechanism and interaction of MEMO1 and IGF-IR in the regulation of invasion and metastasis in gastric cancer cell lines,and to explore a potential target for clinical treatment in the gastric cancer.Methods:Five different differentiated gastric cancer cell lines (MKN-7,NCI-N87,MGC-803,BGC-823,MKN-74) were selected.Transwell was used to compare migration abilities of five gastric cancer cell lines.Western blot was used to detect the different expression levels of MEMO1,IGF-IR and E-cad.The expressions of EMT markers were detected by Western blot analysis after knockdown of MEMO1 or IGF-IR gene instantaneously in one of MEMO1 & IGF-IR over-expression cell lines,MGC-803.IP assay was used to detect the interaction between MEMO1 and IGF-IR.TCGA database was used to analyze the relationship between mRNA expression level of MEMO1 and IGF-IR and long-term survival in gastric cancer patients.Results:IGF-IR was significantly positively correlated with the expression of MEMO1.The migration abilities of five gastric cancer cells were MKN-7
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| Keywords: | MEMO1 IGF-IR gastric cancer EMT |
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