| miR-21调控程序性细胞死亡4基因的表达对宫颈癌细胞增殖和侵袭能力的影响 |
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| 引用本文： | 贺传勇,吴小莉,解为全,倪 荣,柯凤梅. miR-21调控程序性细胞死亡4基因的表达对宫颈癌细胞增殖和侵袭能力的影响[J]. 现代肿瘤医学, 2020, 0(5): 724-728. DOI: 10.3969/j.issn.1672-4992.2020.05.007 |
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| 作者姓名： | 贺传勇 吴小莉 解为全 倪 荣 柯凤梅 |
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| 作者单位： | 恩施土家族苗族自治州中心医院妇产科，湖北.恩施 445000 |
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| 基金项目： | 湖北省自然科学基金资助项目(编号:2014CFC1066) |
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| 摘 要： | 目的:探讨miR-21调控程序性细胞死亡4基因的表达对宫颈癌细胞增殖和侵袭能力的影响及其机制。方法:qPCR检测miR-21和PDCD4在宫颈癌组织中的表达情况；双荧光素酶实验检测miR-21和PDCD4之间的调控作用；细胞集落实验检测抑制miR-21对宫颈癌细胞增殖能力的影响情况，Transwell侵袭实验检测对宫颈癌细胞侵袭能力的影响情况。结果:与癌旁正常组织相比，miR-21在宫颈癌组织中的表达上调，PDCD4在宫颈癌组织中表达下调；双荧光素酶实验检测miR-21和PDCD4之间的调控关系，miR-21可直接调控PDCD4蛋白的表达及活性情况；抑制miR-21的表达后可以抑制宫颈癌细胞的增殖能力；抑制miR-21的表达水平后，宫颈癌细胞的侵袭能力得到一定程度的抑制。结论:miR-21可以调控PDCD4的表达影响宫颈癌细胞的增殖和侵袭能力。
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| 关 键 词： | miR-21 宫颈癌 PDCD4 肿瘤侵袭 |
Effect of miR-21 on proliferation and invasion of cervical cancer cells through regulating programmed cell death 4 gene expression |
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| He Chuanyong,Wu Xiaoli,Xie Weiquan,Ni Rong,Ke Fengmei. Effect of miR-21 on proliferation and invasion of cervical cancer cells through regulating programmed cell death 4 gene expression[J]. Journal of Modern Oncology, 2020, 0(5): 724-728. DOI: 10.3969/j.issn.1672-4992.2020.05.007 |
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| Authors: | He Chuanyong Wu Xiaoli Xie Weiquan Ni Rong Ke Fengmei |
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| Affiliation: | Department of Obstetrics and Gynecology，Enshi Tujia and Miao Autonomous Prefecture Central Hospital，Hubei Enshi 445000，China. |
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| Abstract: | Objective:To investigate the effect of miR-21 on the expression of apoptosis-related gene 4 (PD-4) on the proliferation and invasion of cervical cancer cells and its mechanism.Methods:qPCR was used to detect the expression of miR-21 and PDCD4 in cervical cancer.Dual luciferase assay detected the regulatory role of miR-21 and PDCD4.Cell colony assay detected the effect of miR-21 on the proliferation of cervical cancer cells.Transwell invasion test detected invasion ability of cervical cancer cells.Results:The expression of miR-21 was up-regulated in cervical cancer and the expression of PDCD4 was down-regulated in cervical cancer compared with adjacent normal tissues.Dual luciferase assay detected the regulatory relationship between miR-21 and PDCD4，miR-21 can directly regulate the expression of PDCD4 protein and activity.Inhibition of miR-21 expression can inhibit the proliferation of cervical cancer cells.After inhibition of miR-21 expression levels，cervical cancer cell invasion ability was inhibited to a certain extent.Conclusion:miR-21 can regulate the expression of PDCD4 in cervical cancer cell proliferation and invasion ability. |
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| Keywords: | miR-21 cervical cancer PDCD4 tumor invasion |
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