Induction of hypoglycaemia in Japanese encephalitis virus infection: the role of T lymphocytes |
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| Authors: | N KHANNA A MATHUR M BHARADWAJ U C CHATURVEDI |
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| Affiliation: | Postgraduate Department of Microbiology, K. G. Medical College, Lucknow, India |
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| Abstract: | We report here development of hypoglycaemia in the convalescent phase of Japanese encephalitis virus (JEV) infection in mice by the induction of antigen-specific Ly1−2+ T cells in the spleen which mediate hypoglycaemia through the generation of soluble T cell hypoglycaemic factor (TCHF). The TCHF acted in a dose-dependent manner and was found to be trypsin-sensitive and thermolabile. It was purified on Superose-12 high performance liquid chromatography (HPLC) gel filtration column and purified protein migrated as a ~25-kD band on SDS–PAGE. The JEV-induced hypoglycaemia coincided with an increased circulating glucagon level, without any alterations in blood insulin and growth hormone concentrations. These effects were mimicked by TCHF. These results indicate that JEV-primed T lymphocytes mediate hypoglycaemia through the production of a soluble hypoglycaemic factor. |
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| Keywords: | hypoglycaemia Japanese encephalitis virus T cell factor T suppressor cell glucagon |
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