Comparative assessment of HIF‐1α and Akt responses in human lung and skin cells exposed to benzo[α]pyrene: Effect of conditioned medium from pre‐exposed primary fibroblasts |
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| Authors: | Olga Mavrofrydi Panagiota Mavroeidi Panagiota Papazafiri |
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| Affiliation: | Division of Animal and Human Physiology, Department of Biology, University of Athens, Ilissia, Athens, Greece |
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| Abstract: | Exposure to atmospheric pollutants has been accused for many adverse health effects. Benzo[α]pyrene (Β[α]Ρ) in particular, the most extensively studied member of pollutants, is implicated in both cancer initiation and promotion. In the present study, we compared the effects of noncytotoxic doses of Β[α]Ρ, between human skin and lung epithelial cells A431 and A549, respectively, focusing on Akt kinase and HIF‐1α, as it is well known that these proteins are upregulated in various human cancers promoting survival, angiogenesis and metastasis of tumor cells. Also, taking into consideration that fibroblasts are involved in cancer progression, we tested the possible modulation of epithelial cell response by paracrine factors secreted by Β[α]Ρ‐treated fibroblasts. Low doses of Β[α]Ρ were found to enhance epithelial cell proliferation and upregulate both Akt kinase and HIF‐1α, with A549 cells exhibiting a more sustained profile of upregulation. It is to notice that, the response of HIF‐1α was remarkably early, acting as a sensitive marker in response to airborne pollutants. Also, HIF‐1α was induced by Β[α]Ρ in both lung and skin fibroblasts indicating that this effect may be conserved throughout different cell types and tissues. Interestingly however, the response of both proteins was differentially modified upon treatment with conditioned medium from Β[α]Ρ‐exposed fibroblasts. This is particularly evident in A459 cells and confirms the critical role of intercellular and paracrine factors in the modulation of the final response to an extracellular signal. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1103–1112, 2016. |
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| Keywords: | air pollution benzo[α ]pyrene Akt kinase hypoxia‐inducible factor 1 primary fibroblasts |
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