Indium acetate toxicity in male reproductive system in rats |
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| Authors: | Kuo‐Hsin Lee Hsiu‐Ling Chen Chung‐Man Leung Hsin‐Pao Chen Ping‐Chi Hsu |
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| Affiliation: | 1. Department of Safety, Health and Environmental Engineering, National Kaohsiung First University of Science and Technology, Kaohsiung, Taiwan;2. Department of Emergency Medicine, E‐Da Hospital, I‐Shou University, Kaohsiung, Taiwan;3. Institute of Occupational Safety and Hazard Prevention, Hung Kuang University, Taichung, Taiwan;4. Department of Radiation Oncology, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan;5. Department of Surgery, E‐DA Hospital, I‐Shou University, Kaohsiung, Taiwan;6. School of Health Sciences, Faculty of Health and Medicine, The University of Newcastle, Callaghan NSW, Australia |
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| Abstract: | Indium, a rare earth metal characterized by high plasticity, corrosion resistance, and a low melting point, is widely used in the electronics industry, but has been reported to be an environmental pollutant and a health hazard. We designed a study to investigate the effects of subacute exposure of indium compounds on male reproductive function. Twelve‐week old male Sprague‐Dawley rats were randomly divided into test and control groups, and received weekly intraperitoneal injections of indium acetate (1.5 mg/kg body weight) and normal saline, respectively, for 8 weeks. Serum indium levels, cauda epididymal sperm count, motility, morphology, chromatin DNA structure, mitochondrial membrane potential, oxidative stress, and testis DNA content were investigated. The indium acetate‐treated group showed significant reproductive toxicity, as well as an increased percentage of sperm morphology abnormality, chromatin integrity damage, and superoxide anion generation. Furthermore, positive correlations among sperm morphology abnormalities, chromatin DNA damage, and superoxide anion generation were also noted. The results of this study demonstrated the toxic effect of subacute low‐dose indium exposure during the period of sexual maturation on male reproductive function in adulthood, through an increase in oxidative stress and sperm chromatin DNA damage during spermiogenesis, in a rodent model. © 2014 Wiley Periodicals, Inc. Environ Toxicol 31: 68–76, 2016. |
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| Keywords: | indium acetate serum indium level male reproductive toxicity sperm function sperm DNA damage |
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