| 蒿甲醚对人鼻咽癌细胞CNE-1放射敏感性的影响及其机制研究 |
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| 引用本文： | 潘麓羽,曹建平,纪蓉,封阳,周媛媛,彭晓梅,刘杨,陈遐林,崔丹,朱巍,樊赛军. 蒿甲醚对人鼻咽癌细胞CNE-1放射敏感性的影响及其机制研究[J]. 中华放射医学与防护杂志, 2010, 30(4): 550-553. DOI: 10.3760/cma.j.issn.0254-5098.2010.05.013 |
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| 作者姓名： | 潘麓羽 曹建平 纪蓉 封阳 周媛媛 彭晓梅 刘杨 陈遐林 崔丹 朱巍 樊赛军 |
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| 作者单位： | 江苏省放射医学与防护重点实验室,苏州大学医学部放射医学与公共卫生学院,215123;江苏省吴江市疾病预防控制中心;苏州市市立医院东区,肿瘤内科实验室;绍兴市人民医院; |
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| 基金项目： | 国家自然科学基金教育部长江学者创新团队项目 |
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| 摘 要： | Objective To evaluate the effect of artemether on the cell cycle and the radiosensitivity in human nasopharyngeal carcinoma cell line CNE-1.Methods Cell growth inhibition was assessed with MTT.The method of colony-forming was used to detect the radiation sensitivity.Cell cycle distribution was analyzed by using flow cytometry.The protein expressions of clyclin B1 and Weei were detected by using Western blot.Results The growth of CNE-1 cells was inhibited in a dose-dependent manner.The concentration of 20 μmol/L artemether had radiosensitive effect on CNE-1 cells at 24 h after administration,and SER was 1.481.When CNE-1 cell was irradiated,the G2/M cells increased (t =4.59,P ＜ 0.05).After exposure to combination of artemether and irradiation,the G2/M cells were decreased (t= 10.60,P ＜ 0.05).Western blot showed that artemether increased the level of cyclin B1 expression and inhibited the level of Weel expression.Conclusions The noncytotoxic concentration of artemether could enhance radiosensitization of CNE-1 cells.The radiosensitivity enhancement of artemether might depend on the exposure time.The effect is most obvious when radiation is delivered 24 h after expose to artemetherr.The radiosensitizing effect could be related to apoptosis.
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| 关 键 词： | 蒿甲醚 鼻咽癌 放射敏感性 |
Effect of artemether on radiosensitivity in human nasopharyngeal carcinoma cell line CNE-1 |
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| PAN Lu-yu,CAO Jian-ping,JI Rong,FENG Yang,ZHOU Yuan-yuan,PENG Xiao-mei,LIU Yang,CHEN Xia-lin,CUI Dan,ZHU Wei,FAN Sai-jun. Effect of artemether on radiosensitivity in human nasopharyngeal carcinoma cell line CNE-1[J]. Chinese Journal of Radiological Medicine and Protection, 2010, 30(4): 550-553. DOI: 10.3760/cma.j.issn.0254-5098.2010.05.013 |
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| Authors: | PAN Lu-yu CAO Jian-ping JI Rong FENG Yang ZHOU Yuan-yuan PENG Xiao-mei LIU Yang CHEN Xia-lin CUI Dan ZHU Wei FAN Sai-jun |
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| Abstract: | |
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| Keywords: | ArtemetherNasopharyngeal neoplasmsRadiosensitivity |
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